新型细菌菌体PS2的表征和应用,用于控制不同食品矩阵中的致病性大肠杆菌
Song Zhang1, Kanghee Ryu2, Jin-Chul Kim1
1Department of Biomedical Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea.
International journal of food microbiology
|July 2, 2025
概括
这项研究引入了Escherichia菌体PS2,一种新型的生物控制剂,有效对抗各种大肠杆菌血清型. 菌体PS2表现出稳定性和强烈的化活性,通过减少各种食品模型中的细菌种群来提高食品安全.
科学领域:
- 微生物学和病毒学
- 食品科学与技术 食品科学与技术
- 生物技术是生物技术.
背景情况:
- 大肠杆菌 (E. coli) 由于其多种致病菌株,造成了重大食品安全风险.
- 新型抗微生物策略对于控制食品中的大肠杆菌污染至关重要.
- 菌体为控制病原体提供了传统抗生素的有希望的替代品.
研究的目的:
- 为了描述一种新的埃舍里希亚菌体,vB_EcoS_PS2,其作为生物控制剂的潜力.
- 评估菌体PS2对致病性大肠杆菌的稳定性,菌活性和疗效.
- 评估菌体PS2在各种食品矩阵中减少大肠杆菌的应用.
主要方法:
- 隔离和全基因组分析的埃舍里希亚菌体vB_EcoS_PS2.
- 在不同温度和pH值范围内评估菌体稳定性.
- 在液体,生物膜和食品模型 (牛奶,大米,香肠) 中对大肠杆菌血清型的生物控制疗效的评估.
主要成果:
- 菌体PS2的基因组没有抗生素耐药性,溶解性和毒性基因.
- 它表现出高光活性,短暂的潜伏期和大爆发大小.
- 菌体PS2在牛奶,大米和香肠中显著减少了大肠杆菌种群,有效性受到感染多重性的影响 (MOI).
结论:
- 埃舍里希亚菌体PS2是一种稳定,强大的菌体,对致病性大肠杆菌具有广泛的疗效.
- 它降低细菌负载和消除生物膜的能力使其成为食品安全应用的有希望的候选人.
- 优化的应用策略是必要的,以最大限度地提高复杂的食品系统中菌体PS2的疗效.
相关概念视频
01:28Biological Methods for Microbial Control
214
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
214
00:43Lysogenic Cycle of Bacteriophages
63.4K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
63.4K
01:26DNA Bacteriophages
162
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
162
01:30Lytic Cycle of Bacteriophages
72.2K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
72.2K
01:20Viral Replication: Lytic Cycle
293
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
293