利安凯因配方通过NF-κB/NLRP3通路调节M1巨细胞极化来缓解反流性喉炎
Lirong Wang1, Xuqing Chen2, Tianyu Xu1
1Department of Otolaryngology, Jiangsu Provincial Second Chinese Medicine Hospital(The Second Affiliated Hospital of Nanjing University of Traditional Chinese Medicine), Nanjing 210017, Jiangsu Province, China.
Immunobiology
|July 2, 2025
概括
利安凯因配方 (LYKYF) 通过抑制M1巨细胞两极分化,缓解了老鼠的逆流喉炎. 这通过抑制核因子-κB (NF-κB) /节点状受体蛋白3 (NLRP3) 途径发生,减少炎症.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
背景情况:
- 逆流性喉炎是一种因胃酸逆流而引起的喉炎症的情况.
- M1巨细胞两极分化在与反流炎相关的炎症过程中起着至关重要的作用.
研究的目的:
- 在大鼠模型中研究Liyan Kaiyin配方 (LYKYF) 对逆流喉炎的治疗效果.
- 阐明涉及M1巨分化和核因子-κB (NF-κB) /节点状受体蛋白3 (NLRP3) 途径的潜在机制.
主要方法:
- 患有喉回流病 (LPRD) 的老鼠被用LYKYF.治疗.
- 使用ELISA测量血清炎症标记物 (IL-6,IL-1β,TNF-α).
- 与NF-κB/NLRP3通路和M1巨标记物 (CD86,iNOS) 相关的蛋白质和mRNA的表达,通过西方斑点和RT-PCR进行了分析.
主要成果:
- LPRD诱导炎症性细胞因子,M1巨细胞标记物和NF-κB/NLRP3通路组件的显著增加.
- 与LPRD组相比,LYKYF治疗显著降低了这些升高的标志物.
- 基因丰富分析显示,它与信号转导和炎症反应有关.
结论:
- 在老鼠中,LYKYF对逆流喉炎具有治疗效果.
- 该机制涉及通过抑制NF-κB/NLRP3信号通路来抑制M1巨细胞极化.
- LYKYF具有作为逆流喉炎治疗的潜力.
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