相关实验视频

Updated: Sep 17, 2025

A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia

A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia

Published on: September 16, 2017

磁共振成像在清醒的小鼠中测量大脑血液输液

Ahmed A Bahrani¹, Stephen R Dundon², TianYu Gao²

¹Department of Neurology, University of Kentucky, Lexington, KY, United States; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY, United States.

Journal of neuroscience methods

|July 2, 2025

概括

麻醉在小鼠中显著提高脑血 perfusion (CBP),影响研究的准确性. 我们开发了一种新的MRI设置用于清醒的小鼠成像,使神经成像研究更可靠.

科学领域:

神经成像是一种神经成像.
脑血管生理学脑血管生理学
动物模型动物模型

背景情况:

脑血 perfusion (CBP) 对于大脑功能至关重要,经常使用MRI在小鼠中进行研究.
在这些研究中常用的麻醉剂显著改变了脑血管动力学和代谢活动,这对准确的研究构成了挑战.
在生理条件下了解CBP对于推进神经成像研究至关重要.

研究的目的:

开发和验证一种测量脑血 perfusion (CBP) 的方法在醒着的小鼠使用MRI.
为了比较清醒和麻醉小鼠的CBP测量,以评估麻醉的影响.
为研究非麻醉动物模型中的大脑功能提供可靠的神经成像技术.

主要方法:

开发了一种定制设计的3D打印头部稳定系统,与MRI兼容.
使用流量敏感的交替逆转回收 (FAIR) 序列来测量CBP.
一只野生型老鼠接受了为期三天的训练,以适应MRI环境,以便在清醒时进行扫描.

主要成果:

麻醉小鼠的脑血 perfusion (CBP) 在整个大脑和皮层 (P < 0.001) 的醒着小鼠相比,在麻醉小鼠显著更高.
平均全脑输液在麻醉下为70.9±5.6毫升/分钟/100克,在清醒状态下为51.1±3.3毫升/分钟/100克.
输液可变性,以差异和标准偏差表示,在麻醉下始终更高,这表明可复制性降低.

结论:

麻醉显著增加CBP,损害了输液相关的神经成像研究的准确性,可重现性和相关性.
一个实用的,MRI兼容的设置,用于成像醒着的小鼠成功开发和使用.
这种新方法通过在生理,清醒状态下测量输液,使得神经成像研究更加可靠.

关键词:

3D打印的鼠标头部固定架麻醉麻醉是一种麻醉.大脑的血液流动.脑血管疾病脑血管疾病流量敏感的交替逆转回收 (FAIR)磁力共振成像扫描 (MRI) 扫描鼠标的适应方式没有麻醉的动物.

更多相关视频

Middle Cerebral Artery Occlusion Allowing Reperfusion via Common Carotid Artery Repair in Mice

Middle Cerebral Artery Occlusion Allowing Reperfusion via Common Carotid Artery Repair in Mice

Published on: January 23, 2019

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

Published on: September 20, 2015

相关概念视频

Magnetic Resonance Imaging

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...