Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Plasmids01:28

Plasmids

152
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
152
Genome Copying Errors02:46

Genome Copying Errors

4.4K
DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their  survival. Therefore, the copying errors are checked and repaired at three levels.
4.4K
Replication in Prokaryotes01:32

Replication in Prokaryotes

25.4K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
25.4K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

18.0K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
18.0K
Replication in Eukaryotes02:31

Replication in Eukaryotes

172.5K
Overview
172.5K
The Replisome03:01

The Replisome

35.0K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
35.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ecogenomic kinetics: wandering bacterial populations stumbling upon niches.

Trends in microbiology·2026
Same author

The antimicrobial gut resistome of the Wayampi reveals a shared background of antibiotic and metal resistance genes with industrialized populations, underscoring the "robust-yet-fragile" architecture of human gut microbiomes.

Microbiome·2026
Same author

Plasmid mutation rates scale with copy number.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Abnormal Splicing of <i>GALC</i> Transcripts Underlies Unusual Cases of Krabbe Disease.

Biomedicines·2025
Same author

Chagas disease induces gut microbial metabolic stress: Disruption of energy and nucleotide pathways and partial reversal by antiparasitic therapy (TRIPOBIOME-2 study).

Travel medicine and infectious disease·2025
Same author

ExcludonFinder: mapping transcriptional overlaps between neighboring genes.

Nucleic acids research·2025
Same journal

Unlocking the capacity of Mn-based Prussian blue cathodes in capacitive deionization.

Nature communications·2026
Same journal

Scaling biodiversity-stability relationships from populations to meta-communities across trophic levels.

Nature communications·2026
Same journal

Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.

Nature communications·2026
Same journal

Engineering all-organic electrocatalysts with asymmetric dual-active sites for uncommon oxygen-evolving pathway.

Nature communications·2026
Same journal

Rapid GC content evolution in rice through GC-biased gene conversion and selection for translation efficiency.

Nature communications·2026
Same journal

Declines in organic matter persistence with increased soil carbon.

Nature communications·2026
查看所有相关文章

相关实验视频

Updated: Sep 17, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K

全球规则统治着等离子体的复制号码.

Paula Ramiro-Martínez1,2, Ignacio de Quinto1, Val F Lanza1,3

  • 1Microbiology Department, Hospital Universitario Ramón y Cajal-IRYCIS, Madrid, Spain.

Nature communications
|July 2, 2025
PubMed
概括
此摘要是机器生成的。

这项研究表明,细菌等离子体拷贝数 (PCN) 是高度可变但强大的,与等离子体生活方式密切相关. 一个通用缩放定律将PCN和等离子体大小跨物种连接起来.

更多相关视频

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
09:23

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

2.0K
High-Resolution Comparison of Bacterial Conjugation Frequencies
05:18

High-Resolution Comparison of Bacterial Conjugation Frequencies

Published on: January 10, 2019

10.9K

相关实验视频

Last Updated: Sep 17, 2025

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K
Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics
09:23

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

2.0K
High-Resolution Comparison of Bacterial Conjugation Frequencies
05:18

High-Resolution Comparison of Bacterial Conjugation Frequencies

Published on: January 10, 2019

10.9K

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 等离子体,或自主复制的DNA分子,拥有复制,移动性和宿主相互作用的各种策略.
  • 在细菌中决定等离子体拷贝数 (PCN) 的因素尚不清楚.
  • 了解PCN对于各种应用至关重要,包括生物技术和打击抗生素耐药性.

研究的目的:

  • 在广泛的细菌等离子体中全面分析等离子体拷贝数 (PCN) 的决定因素.
  • 研究PCN,等离子体生活方式和基因组背景之间的关系.
  • 确定控制等离子体大小和拷贝数之间的权衡的通用原则.

主要方法:

  • 来自数千种不同的细菌等离子体的广泛DNA测序数据的分析.
  • 统计分析以将PCN与等离子体特征和生活方式相关联.
  • 开发和应用一个通用缩放规律来建模PCN大小关系.

主要成果:

  • 等离子体拷贝数 (PCN) 具有很高的可变性 (大小三级),并且对基因组上下文的变化有很强的抵抗力.
  • PCN与不同的等离子体生活方式密切相关.
  • 一个普遍的缩放定律表明,在细菌物种中,等离子体大小和拷贝数之间存在着有限的权衡.

结论:

  • 等离子体拷贝数受生活方式的影响,并受普遍缩放规律的影响.
  • "复制品优势"的概念有助于解释多复制品等离子体中的相互作用.
  • 普遍的生物约束调节了塑体大小和细菌拷贝数之间的关系.