通过其mRNA相互作用,SIRT1反意义的长非编码RNA通过其mRNA相互作用减弱了人体软骨细胞中interleukin-1β诱导的骨关节炎基因表达
Takeo Tokura1, Takehiko Matsushita2, Kyohei Nishida1
1Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan.
Scientific reports
|July 2, 2025
概括
赛尔图因1反感应长非编码RNA (SIRT1 AS lncRNA) 抑制了软骨细胞中的软骨降解酶. 在骨关节炎中减少的SIRT1 AS lncRNA表明它是潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 基因规则 基因规则
- 在RNA生物学,RNA生物学.
背景情况:
- 反意义长非编码RNAs (AS lncRNAs) 调节基因表达.
- 赛尔图因1 (SIRT1) 在细胞过程中发挥作用.
- 干白素-1β (IL-1β) 涉及到状细胞的功能.
研究的目的:
- 研究SIRT1 AS lncRNA在人类红细胞中IL-1β诱导的基因表达中的作用.
- 探索SIRT1 AS lncRNA在骨关节炎 (OA) 中的治疗潜力.
主要方法:
- 实时PCR用于基因表达分析.
- 西方涂抹用于蛋白质水平评估.
- 核糖核糖酶保护试验用于RNA结合的确认.
主要成果:
- SIRT1 AS lncRNA过度表达抑制了IL-1β诱导的ADAMTS-5和MMP-13的上调调节.
- 抑制SIRT1 AS lncRNA加剧了这种上调.
- 与正常软骨相比,SIRT1 AS lncRNA表达在OA软骨中减少.
结论:
- SIRT1 AS lncRNA与SIRT1 mRNA结合,并抑制IL-1β诱导的软骨降解酶.
- SIRT1 AS lncRNA代表了骨关节炎的潜在治疗点.
相关概念视频
02:15Experimental RNAi
6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
02:30siRNA - Small Interfering RNAs
17.0K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.0K
01:29Translational Regulation
108
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
108
01:23RNA Interference
26.5K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.5K