黄素通过Notch1通路激活来缓解高葡萄糖诱导的心脏氧化应激
Xia Wu1,2, ZhuangYin Qu1,2, PeiYan Liu3,4
1Department of Pharmacy, Nanjing First Hospital, Nanjing Medical University, No. 68 Chang Le Road, Qinhuai District, Nanjing, 210006, Jiangsu, P. R. China.
Scientific reports
|July 2, 2025
概括
黄素 (CUR) 通过减少氧化应激和亡,保护心脏细胞免受高葡萄糖损伤. 它激活了Notch1信号通路,改善了糖尿病患者的细胞活力和抗氧化功能.
科学领域:
- 心脏病学 心脏病学
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 糖尿病与心血管并发症的增加有关,包括糖尿病心肌病.
- 高水平的葡萄糖 (HG) 诱导氧化应激和心肌细胞的亡,导致心脏功能障碍.
- 黄素 (CUR) 是一种天然的多,具有抗氧化,抗亡和抗炎性质,具有潜在的治疗益处.
研究的目的:
- 研究黄素 (CUR) 对高葡萄糖 (HG) 诱导的氧化应激和初级心肌细胞的亡的保护作用.
- 阐明Notch1信号通路在调解CUR的保护作用中的作用.
主要方法:
- 初级新生儿大鼠心肌细胞被暴露在高葡萄糖 (HG) 条件下.
- 细胞被用黄素 (CUR) 和DAPT (一种Notch抑制剂) 治疗.
- 细胞活力,细胞亡,活性氧物种 (ROS) 生产,抗氧化酶活性和Notch1/Hes1信号通路组件 (基因和蛋白质表达) 通过CCK-8,流细胞计,光标记,qRT-PCR和Western blot进行了评估.
主要成果:
- HG暴露显著增加了心肌细胞亡和ROS产生,同时降低了抗氧化酶活性和Notch1/Hes1表达.
- 黄素预治疗显著改善了心肌细胞活力,抑制了细胞亡和ROS产生,并增加了抗氧化酶活性.
- 黄素治疗提高了Notch1和Hes1蛋白和相关基因的表达,这表明Notch1/Hes1信号通路的激活.
结论:
- 黄素对高葡萄糖诱导的氧化应激和心肌细胞的亡具有保护作用.
- 这些保护作用至少部分通过激活Notch1/Hes1信号通路来介导.
- 黄素作为治疗糖尿病心肌病的治疗药物具有潜力.
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