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快速全彩串行切割断层扫描用斑点照明和紫外线激发

Wentao Yu¹, Yan Zhang¹, Claudia T K Lo¹

¹Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China.

|July 3, 2025

概括

高低频 (HiLo) 显微镜与翻译快速紫外激发切割断层扫描 (TRUST) 结合,显著提高了3D成像分辨率. 这种HiLoTRUST方法可以为组织学应用程序提供经济高效,全彩色,高分辨率的全器官成像.

科学领域:

生物医学成像学生物医学成像学
光学显微镜是一种光学显微镜.
历史学历史学历史学

背景情况:

生物组织的三维 (3D) 高分辨率,大体积成像带来了重大挑战.
现有的方法,如转化快速紫外激发切割断层扫描 (TRUST) 提供成本效益高的全器官成像,但由于机械切割厚度或紫外线透深度,轴分辨率受到限制.

研究的目的:

为了提高TRUST的轴分辨率和成像能力,用于全器官3D成像.
为生物标本开发一种具有成本效益,全彩,高分辨率的3D成像方法.

主要方法:

高低频 (HiLo) 显微镜与TRUST (HiLoTRUST) 的集成.
将光学切割厚度降低到5.8微米.
与TRUST (50微米) 相比,实现了更细的机械切割厚度 (10-15微米).
利用额外的紫外线发光二极管 (LED) 在高内容成像中实现均照明.

主要成果:

HiLoTRUST显著降低了光学切割厚度,改善了轴分辨率.
实现了更精细的机械切割,进一步增强了3D成像细节.
验证了对小鼠器官和人类肺癌标本的全彩2D/3D成像.
证明了具有成本效益,高分辨率的3D成像能力.

结论:

HiLoTRUST克服了TRUST的轴分辨率限制.
这种技术提供了一个具有成本效益,全彩色,高分辨率的3D成像解决方案.
HiLoTRUST显示了3D组织学和大容量生物成像应用的巨大潜力.

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