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相关概念视频

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

11.1K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
11.1K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

11.9K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.9K

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相关实验视频

Updated: Sep 17, 2025

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
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在SNARE拆卸时,需要Sec18/NSF侧载荷.

Yousuf A Khan1,2,3,4, K Ian White5,6,7,8,9, Richard A Pfuetzner5,6,7,8,9

  • 1Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA. yousuf@stanford.edu.

Nature structural & molecular biology
|July 3, 2025
PubMed
概括
此摘要是机器生成的。

在细胞膜融合后,Sec18/NSF (N-乙烯胺胺敏感因子) 和Sec17/α-SNAP将SNARE蛋白束分解. 新的冷-EM结构显示Sec18/NSF线索通过其ATPase环通过SNAREs,使侧载和卸载成为可能.

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相关实验视频

Last Updated: Sep 17, 2025

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科学领域:

  • 细胞生物学 细胞生物学
  • 分子机器的分子机器.
  • 膜融合是什么? 膜融合是什么?

背景情况:

  • 通过形成四螺旋,SNARE蛋白调解了膜融合.
  • 在Sec18/NSF和Sec17/α-SNAP中将这些捆拆为回收使用.
  • 之前的模型面临着SNARE跨膜域的拓挑战.

研究的目的:

  • 通过Sec18/NSF阐明SNARE复合体拆解的机制.
  • 解决SNARE通过Sec18/NSF线程的拓约束.
  • 为了可视化Sec18/NSF和Sec17/α-SNAP与SNAREs的相互作用.

主要方法:

  • 酵母SNARE复合物的冷电子显微镜 (冷EM).
  • 质谱测量以确定蛋白质相互作用.
  • 在非水解和水解条件下进行结构分析.

主要成果:

  • 化EM结构显示SNARE Sso1通过Sec18/NSF的D1和D2 ATPase环进行线索.
  • Sso1 的 N-终端 Habc 域与 D2 环相互作用,并保持折叠.
  • 在水解条件下的结构显示基质释放和环开放.
  • 在Sec18/NSF中,用于拓上受约束的SNARE,使用侧面加载和卸载.

结论:

  • Sec18/NSF通过侧载机制将SNARE拆卸.
  • Sec18/NSF的ATPase环开放以容纳和释放SNARE基质.
  • 这种机制克服了SNARE域所带来的拓约束.