H3K4脱甲基酶SsJMJ11负面调节了甘的干旱耐受性反应
Guangrun Yu1,2, Xiaoge Wu1, Meiling Ye1
1Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
一种H3K4me3脱甲基酶SsJMJ11通过降低H3K4me3水平来负面调节甘的干旱压力,影响关键的干旱耐受性基因.
科学领域:
- 植物生物学 植物生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 压力生理学 压力生理学
背景情况:
- 干旱压力显著影响植物基因表达,通常涉及到像H3K4me3.3.这样的基因组修饰的改变.
- 基因组甲基化恒温对于植物应激反应至关重要,并依赖于甲基转移酶和脱甲基酶的平衡.
- 以前,SsJMJ11被确定为Saccharum spontaneum中的H3K4me3脱甲基酶,它参与了花期调节.
研究的目的:
- 调查H3K4me3脱甲基酶SsJMJ11在调节甘 (Saccharum spp. ) 的情况.
- 阐明SsJMJ11影响干旱耐受性的分子机制.
主要方法:
- 在Arabidopsis thaliana中SsJMJ11的宫外过度表达,以评估干旱和酸 (ABA) 敏感性.
- 在过度表达SsJMJ11的Arabidopsis中分析干旱诱导的基因表达 (AtRD20,AtDREB2A).
- 在Saccharum spontaneum原生体中研究了ABA/脱水诱导的基因转录 (SsRD20,SsDREB2A),H3K4me3水平和SsJMJ11活性之间的相关性.
主要成果:
- 在Arabidopsis中SsJMJ11的过度表达导致对干旱,ABA和曼尼托尔的过敏,并抑制了干旱耐受性基因 (AtRD20,AtDREB2A).
- 在S. spontaneum中,ABA和脱水治疗增加了SsRD20和SsDREB2A位点的H3K4me3水平,与它们的转录相关.
- 在S. spontaneum原生体中SsJMJ11的过度表达减少了ABA诱导的SsRD20和SsDREB2A的转录,伴随着H3K4me3水平的降低.
结论:
- 在甘中,SsJMJ11作为干旱应激反应的负调节剂.
- 由SsJMJ11介导的H3K4me3的动态沉积对于甘适应干旱压力至关重要.
- 通过调节关键应激反应基因的表观遗传调节,SsJMJ11影响干旱耐受性.
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