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Updated: Jun 23, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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在克隆的体细胞混合体中,两种人类淋巴细胞细胞系之间的双重免疫球蛋白产生

A Rosén, G Clements, G Klein

    Cell
    |May 1, 1977
    PubMed
    概括

    拉吉和纳马尔瓦之间的体细胞混合体人类淋巴细胞系研究了免疫球蛋白表达. 大多数杂交克隆共表达了卡帕和羊羔光链,表明稳定的表面免疫球蛋白表达.

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    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...