使用新建的细菌联盟,有效地生物降解和排毒反应性黑色5
Manar K Abd Elnabi1,2, Mohamed A Ghazy1,3, Sameh S Ali4
1Biotechnology Program, Basic and Applied Science Institute, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City, Alexandria, 21934, Egypt.
Microbial cell factories
|July 3, 2025
概括
一个新的细菌联盟有效地去色和排毒了反应性黑色5 (RB5) 染料,达到98.56%的效率. 这种环保方法显著降低了染料毒性,为织废水处理提供了可持续的解决方案.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 废水处理 废水处理
背景情况:
- 亚染料,特别是反应性黑5 (RB5),是织工业产生的持续性环境污染物.
- 它们的复原性和毒性对废水整治构成重大挑战.
研究的目的:
- 开发和优化一个细菌联盟,以有效地去色和RB5.5的排毒.
- 评估降解产品的环境安全性.
主要方法:
- 分离和结合Bacillus cereus,Proteus mirabilis和Stenotrophomonas maltophilia,形成一个联盟.
- 使用Plackett-Burman设计 (PBD) 和中央复合设计 (CCD) 优化培养条件.
- 酶分析,代谢物表征 (UV-Vis,FTIR,GC-MS) 和生态毒性评估.
主要成果:
- 在静态条件下,优化的联盟实现了98.56%的RB5脱色.
- 确定了关键酶:NADH-DCIP减少酶和阿佐减少酶用于亚键裂解.
- 毒性显著降低:根生长抑制 (66.38-21.38%),发芽增加 (40-93.33%),Artemia salina死亡率降低 (86.7-23.3%),在人类细胞中细胞毒性降低 (55.31-14.45%).
结论:
- 开发的细菌联盟为处理RB5污染的废水提供了一种高效,环保和具有成本效益的方法.
- 降解产生无毒代谢物,减轻环境风险.
- 工业应用的潜力等待试点规模研究和稳定性评估.
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