新型非编码FOXP3转录异型与人类调节性T细胞的潜在转录干扰相关
Marcos Cases1, Niclas Ritter2, Hector Rincon-Arevalo3,4,5
1Flow Cytometry Core Facility, Deutsches Rheuma-Forschungszentrum Berlin, A Leibniz Institute (DRFZ), Berlin, Germany.
RNA biology
|July 3, 2025
概括
在调节性T细胞中 (TREGS) 新发现的FOXP3基因替代促进体产生非编码的转录. 这种替代性促进剂可能会降低FOXP3水平,影响免疫耐受性和TREGS功能.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- CD4+调节性T细胞 (TREGS) 对于免疫耐受性至关重要.
- 转录因子叉头盒P3 (FOXP3) 对TREGS的分化和功能至关重要.
- 一个在TREGS中活跃的替代FOXP3促进体可能会改变基因输出和细胞功能.
研究的目的:
- 为了研究人类FOXP3位点的转录复杂性.
- 描述新型FOXP3转录产品及其功能影响.
- 在TREGS中探索替代FOXP3促进者的作用.
主要方法:
- 直接RNA测序以识别新的FOXP3转录.
- 西方涂抹和质谱法用于评估蛋白质产品.
- RNA单分子光在现场杂交以分析转录本地化和水平.
- 在体外转录和转染实验.
主要成果:
- 识别多个新型FOXP3转录,包括一个非编码异型"longFOXP3",具有扩展的5' UTR.
- 有证据表明"longFOXP3"不会转化为蛋白质.
- 从替代上游FOXP3促进体转录与减少的FOXP3mRNA和蛋白质水平相关.
- 证明了替代FOXP3促进剂和正规FOXP3促进剂之间的内基因转录干扰.
结论:
- 人类FOXP3位点表现出比以前注释的更大的转录复杂性.
- 替代FOXP3促进体及其非编码转录"longFOXP3"可以通过转录干扰微调TREGS中的FOXP3水平.
- 需要进一步的研究,以充分识别和描述所有编码和非编码FOXP3异型及其在免疫调节中的作用.
相关概念视频
General Transcription Factors
5.6K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.6K
Master Transcription Regulators
7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
lncRNA - Long Non-coding RNAs
9.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Transcription Factors
77.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
77.1K
Inheritance of Chromatin Structures
6.6K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.6K
Regulation of Expression at Multiple Steps
1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.0K


