综合性基因组和转录组分析识别了葡萄藤果中对烟雾反应敏感的糖转移酶
Manon Paineau1, Chen Liang1, Noe Cochetel1
1Department of Viticulture and Enology, University of California Davis, Davis, CA, 95616, USA.
Journal of experimental botany
|July 3, 2025
概括
野火威胁葡萄树,造成烟雾污染. 研究人员在Pixie葡萄树中发现了12个对烟雾有反应的关键基因 (VviSIUGT),为开发耐烟葡萄品种提供了目标.
科学领域:
- 植物遗传学 植物遗传学
- 葡萄种植 葡萄种植 葡萄种植
- 分子生物学分子生物学
背景情况:
- 野火对葡萄栽培构成重大威胁,导致葡萄因烟雾衍生的挥发性而受到烟雾污染.
- 烟雾污染导致不良风味,导致葡萄酒行业的经济损失.
- 葡萄糖转移酶 (GTs) 将挥发性代谢成稳定的葡萄糖合物,这是烟雾污染形成的关键步骤.
研究的目的:
- 建立一个模型系统来研究葡萄园中的烟雾污染.
- 为了确定参与葡萄树对烟雾暴露的反应的基因.
- 发现分子标记物和潜在的目标,用于培育耐烟葡萄品种.
主要方法:
- 使用HiFi长读测序,为Pixie和Pinot Noir生成了哈普洛型解析的端粒到端粒双倍基因组.
- 为Pixie创建了跨越各种组织和发育阶段的表达图谱.
- 进行了受控的烟雾暴露实验,分析基因表达和测量挥发性.
主要成果:
- 确定了12个GT1基因,被命名为Vitis vinifera烟雾诱导性UGTs (VviSIUGTs),因暴露于烟雾而显著上调.
- 暴露后一天观察到峰值VviSIUGT表达,在第三天下降,与糖化物积累相关.
- 基因共同表达网络和转录因子结合位点分析突出了应激响应的调节元素和模块.
结论:
- 发现葡萄暴露在烟雾中的早期转录反应.
- 确定了VviSIUGTs作为分子标记物或工程目标的候选基因,以减少烟雾污染易感性.
- 皮克西葡萄藤品种作为未来烟雾污染研究的有价值的模型.
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