由于免疫试验干扰而引起的明显高雄性质血症通过液体染色学-并联质谱学解决
Dongni Huang1,2, ZhiXuan Guo3, JingWen Fan3
1Department of Endocrinology, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing 100730, China.
JCEM case reports
|July 3, 2025
概括
免疫测试干扰可能会导致女性丸激素水平虚假高. 液体染色学-并联质谱学 (LC-MS/MS) 证实了正常水平,确定异性恋抗体为病因.
科学领域:
- 临床化学 临床化学
- 内分泌学 在内分泌学.
- 分析生物化学 分析生物化学
背景情况:
- 免疫测试是临床丸激素测量的标准,但容易产生分析干扰.
- 女性生理学上较低的丸激素意味着轻微的升高可以模仿病理,使诊断复杂化.
研究的目的:
- 为了报告一个女性由于免疫测试干扰导致血清丸激素错误升高的病例.
- 突出这些文物所带来的诊断挑战.
- 强调质谱在解决这些差异方面的实用性.
主要方法:
- 一个43岁的女性持续升高的丸激素的病例报告.
- 进行了全面的内分泌和成像评估.
- 血清丸激素使用液体染色学-并联质谱法 (LC-MS/MS) 重新分析.
- 用蛋白沉试验来识别干扰物质.
主要成果:
- 最初的免疫测试始终显示丸激素水平升高.
- 广泛的检查未能确定雄激素过量的内源源.
- 经过LC-MS/MS检查,确认丸激素水平正常.
- 异构型抗体被确定为免疫试验干扰的原因.
结论:
- 在无法解释的生化过高雄性质症,特别是没有临床症状的情况下,应考虑测试干扰.
- 在怀疑免疫测试干扰时,LC-MS/MS是确认水平的关键工具.
- 了解免疫测试的局限性对于准确的内分泌诊断至关重要.
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