在胃癌瘤组织中发现的活细菌
Xiaoqian Lv1,2, Pengxia Song3, Nianhua Zhang1,4
1Department of Clinical Laboratory Medicine, The first Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Laboratory of Metabolism and Gastrointestinal Tumor, Shandong, China.
Frontiers in microbiology
|July 3, 2025
概括
在胃癌组织中发现了包括Propionibacterium acnes,Klebsiella pneumoniae和Lactobacillus salivarius在内的活细菌. P. acnes 特别对致癌蛋白POF1B进行升级调节,这表明细菌在胃癌的发展中发挥了新的作用.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
背景情况:
- 关于胃癌和微生物的研究主要集中在Helicobacter pylori上.
- 有限的证据直接将胃癌组织中的细菌与疾病联系起来.
研究的目的:
- 研究胃癌组织中细菌的存在和作用.
- 为了确定特定细菌对胃癌细胞和蛋白质表达的影响.
主要方法:
- 传输电子显微镜和格拉姆染色被用来观察和识别胃癌组织中的细菌.
- 高通量16S rRNA测序对细菌群体的特征.
- 在实验室中感染纯细菌培养的AGS胃癌细胞,然后进行西式涂抹,评估POF1B蛋白质表达.
主要成果:
- 在胃癌组织中发现了Propionibacterium acnes,Klebsiella pneumoniae和Lactobacillus salivarius.
- 感染P. acnes导致AGS细胞的真空化和死亡率增加 (p < 0.05).
- 在AGS细胞中,P. acnes显著上调POF1B蛋白表达 (p < 0.05).
结论:
- 活体细菌,包括L. salivarius,P. acnes和K. pneumoniae,存在于胃癌瘤组织中.
- P. acnes 在上调瘤原蛋白POF1B. 的特定作用.
- 需要进一步的研究来阐明细菌进入的机制及其在胃瘤微环境中的功能.
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