连接动脉样硬化与缺血性中风的表观遗传机制:从DNA甲基化和转录组集成的见解
Binrong Ding1, Yiqun Wang2, Junfeng Li1
1People's Hospital of Ningxiang City, Hunan University of Chinese Medicine, Changsha, China.
Frontiers in genetics
|July 3, 2025
概括
表观遗传调节,特别是DNA甲基化,对于将动脉样硬化和缺血性中风联系起来至关重要. 这项研究确定了共享的分子通路,提供了对疾病进展和潜在治疗点的见解.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 心血管科学 心血管科学
背景情况:
- 缺血性中风 (IS) 是导致死亡和残疾的主要原因.
- 动脉样硬化 (AS) 是IS的主要危险因素.
- 表观遗传机制,特别是DNA甲基化,涉及AS病原体,但尚未完全理解.
研究的目的:
- 研究连接AS和IS的表观遗传调节机制.
- 整合DNA甲基化和转录组数据以确定共享的分子途径.
- 发现治疗干预的潜在分子标.
主要方法:
- 综合DNA甲基化 (GSE46394) 和转录组数据 (GSE111782,GSE162955) 来自公共存储库.
- 识别了差异甲基化CpG位置 (DMP) 和差异表达基因 (DEG),使用p值值<0.05.
- 进行基因注释和通路丰富分析以阐明分子机制.
主要成果:
- 在AS病变中确定了5,396个一致的DMP,富含MAPK和Hippo信号通路.
- 揭示了AS斑块中的1,147个DEG和IS脑组织中的1,321个DEG,富含神经活性联体受体相互作用,信号传递和血管光滑肌肉收缩通路.
- 发现了共享的过程,包括行为丝聚合,细胞迁移,MAPK级联调节,上腺体信号传递和阿林信号传递.
结论:
- 表观遗传调节在AS和IS的进展中起着至关重要的作用.
- 确定了关键的分子通路和过程,将AS和IS连接起来.
- 强调需要在更大的队列中进一步验证和多omics集成,以获得全面的理解.
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