用酵母细胞工厂重组生产阿马兰丁和其他贝他林变体
Christiane Glitz1, Jane Dannow Dyekjær1, Gian Maria Cristian Solimando1
1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet Building 220, DK-2800, Kgs. Lyngby, Denmark.
Synthetic and systems biotechnology
|July 3, 2025
概括
这项研究探讨了微生物生产各种贝他林,天然食品染色剂. 研究人员成功地设计了酵母菌株,以生产阿马兰丁及其衍生物,扩大了可持续食品染料的选择.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 植物生物化学 植物生物化学
背景情况:
- 贝塔林是来自植物的天然颜料,贝塔林主导着食品颜色市场.
- 目前正在探索重组生产贝塔宁,但其他贝塔宁变体的研究较少.
- 微生物发酵为植物提取提供了一种可持续的替代品,用于生产食品色素.
研究的目的:
- 通过微生物发酵来研究各种贝塔林变体的重组生产.
- 探索贝塔宁的酶修饰以产生其他有价值的贝塔林.
- 扩大可通过酵母发酵生产的天然食品染料的范围.
主要方法:
- 从各种植物物种中选择的葡萄糖转移酶用于酶选.
- 使用*Saccharomyces cerevisiae*和*Yarrowia lipolytica*作为贝他林生产的宿主菌株.
- 采用料批发发酵来优化阿马兰丁产量和共同表达的酶用于衍生合成.
主要成果:
- 工程酵母菌株从贝坦宁中生产了布根维林-rI和阿马兰丁.
- 在料批发发酵中达到2.97g/L的最大阿玛兰丁产量.
- 通过共同表达葡萄糖基转移酶和一个基转移酶,成功合成了6'-O-基基氨酸.
结论:
- 微生物发酵可以有效地产生超越贝他宁的各种贝他林变体.
- 这项研究扩大了通过生物技术可用的天然食品着色剂的调色板.
- 这项研究有助于了解贝他林生物合成途径,并使新的色素生产成为可能.
相关概念视频
01:09Recombinant DNA
Overview
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:27Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
01:27Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
01:30Production of Pharmaceuticals
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...