聚乙烯纳米塑料加剧剂HFD诱导的MASLD通过减少甲素活性并通过受损的溶酶体酸化触发大真空球形成
Jiwon Ahn1, Kajung Ryu1, Hyerin Kim2
1Center for Gene and Cell Therapy, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
International journal of biological sciences
|July 3, 2025
概括
环境纳米塑料,特别是聚乙烯纳米颗粒 (PS-NPs),在食高脂肪饮食的小鼠中加剧脂肪肝疾病. PS-NP 干扰细胞过程,导致肝脏损伤增加和脂质积累.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 环境纳米塑料 (NP) 对人类健康构成风险.
- 聚乙烯纳米颗粒 (PS-NP) 是常见的环境污染物.
- 脂肪肝疾病,包括与代谢相关的脂肪性肝病 (MASLD),是一个日益严重的健康问题.
研究的目的:
- 研究聚烯纳米颗粒 (PS-NP) 对肥胖症和脂肪肝疾病的影响.
- 阐明通过PS-NPs影响肝细胞的细胞机制.
主要方法:
- 在正常或高脂肪饮食 (HFD) 的小鼠中,口服PS-NP.
- 肝酶和胆固醇的生物化学分析.
- 转录组分析以确定受影响的细胞通路.
- 使用HepaRG细胞进行体外研究,观察NP吸收和细胞效应.
主要成果:
- 在HFD小鼠中,PS-NPs加剧了肥胖症和肝损伤,增加了ALT,AST和胆固醇.
- PS-NPs干扰了行动蛋白组织,细胞-细胞粘附,PPAR信号传递和脂质代谢.
- 在HepaRG细胞中,PS-NPs诱导了内细胞分裂,导致溶酶体功能障碍,蛋白质降解受损 (p62,LC3B),并降低了甲素活性.
- 暴露于PS-NP增加了脂质滴积累,并改变了与脂质发生有关的基因的表达.
结论:
- 特别是在高脂肪饮食的背景下,PS-NP会显著恶化MASLD.
- 由PS-NPs诱导的过度内细胞分裂会破坏溶解体功能,并促进肝细胞中的脂质积累.
- 这些发现凸显了纳米塑料对肝脏健康和代谢过程的有害影响.
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