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功能性料成分调节RTgutGC细胞对LPS诱导的炎症的免疫反应

Malene Malchus Fosse¹, Laura Rivera Méndez², Tania Rodríguez-Ramos²

¹Department of Biology, Norwegian University of Science and Technology, Trondheim, Norway.

Frontiers in immunology

|July 3, 2025

概括

功能性料成分如β-葡萄糖和拉米纳林对鱼的肠道健康有影响. 这项研究评估了它们对彩虹鱼肠道细胞的影响,发现它们调节免疫反应和屏障功能,有助于水产养殖的弹性.

科学领域:

水产养殖营养和免疫学
鱼细胞系模型鱼细胞系模型
功能性料成分的有效性功能性料成分的有效性

背景情况:

水产养殖面临的挑战是鱼的肠道完整性和免疫弹性.
功能性料成分为改善鱼类健康提供了潜在的解决方案.
了解成分的作用需要强大的体外模型.

研究的目的:

评估选定功能性料成分的免疫调节和屏障效应.
在正常和挑战条件下评估成分对彩虹鱼肠细胞 (RTgutGC) 的影响.
验证RTgutGC细胞系作为选料成分的工具.

主要方法:

使用了RTgutGC彩虹鱼肠道细胞系.
暴露于不同度的β-葡萄糖,拉米纳林,卡诺辛和鱼水解盐的细胞.
在脂多糖化物 (LPS) 试验之前和之后评估过体电阻 (TEER),透性 (P_app) 和基因表达 (qPCR).

主要成果:

高剂量的拉米纳林和鱼水解剂增加了促炎性细胞因子mRNA.
大多数成分降低了TEER,而低剂量的肉素和拉米纳林降低了P_app,表明屏障调节.
由LPS引起的炎症和屏障功能障碍被β-葡萄糖和拉米纳林调节;鱼水解剂促进了恢复.

结论:

选择的功能成分对鱼的肠细胞表现出明显的免疫调节和屏障作用.
RTgutGC细胞系是选料成分生物活性的一个有价值的工具.
结果提供了关于成分有效性的见解,以提高鱼的肠道健康和水产养殖中的弹性.

关键词:

土耳其人土耳其人土耳其人土耳其人生物活性类的生物活性.鱼的肠道健康鱼的肠道健康免疫调节免疫调节脂多糖 (LPS) 是一种脂多糖.的β-葡萄糖.

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