相关实验视频

Updated: Jun 18, 2026

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

Published on: March 15, 2012

在斑马鱼脑膜炎模型中,B组链球菌溶解内皮细胞以感染大脑

Sumedha Ravishankar^1,2, Samantha M Tuohey¹, Nicole O Ramos¹

¹School of Biological Sciences, UC San Diego, La Jolla, California, United States of America.

|July 3, 2025

概括

乙组链球菌 (GBS) 通过入侵大脑引起新生儿脑膜炎. 在体内,GBS通过内皮细胞溶解而不是细胞转移突破了血脑屏障,挑战了先前的体外发现.

科学领域:

微生物学微生物学
神经科学是一个神经科学.
传染性疾病传染性疾病

背景情况:

细菌性脑膜炎涉及到穿越血脑屏障 (BBB) 的病原体.
乙组链球菌 (GBS) 是新生儿脑膜炎的主要原因.
之前的研究表明,基于体外研究,GBS通过转细胞化穿越BBB.

研究的目的:

为了研究GBS血脑屏障 (BBB) 透的体内机制.
利用斑马鱼幼虫作为研究细菌性脑膜炎的体内模型.

主要方法:

斑马鱼幼虫中GBS的时间延迟共聚焦显微镜.
细菌内皮细胞相互作用和BBB完整性的分析.
研究GBS lysin CylE和宿主凝固因子的作用.

主要成果:

在大脑血管中形成微殖民地,导致内皮细胞穿孔和溶解.
细菌进入大脑是由BBB损伤促进的,而不是细胞转移.
在体内脑部的入侵中,GBS lysin CylE并不是必不可少的.
在GBS诱导的BBB分解过程中,促炎媒介被上调.

结论:

在体内,GBS通过引起内皮细胞溶解和死亡来侵入大脑,这种机制与转细胞形成不同.
感染血管中的血栓形成可能起到对脑部入侵的保护作用.
观察到的入侵路线在Streptococcus pneumoniae中得到保留,这表明链球菌脑膜炎的更广泛的机制.

更多相关视频

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Published on: June 5, 2019

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Published on: August 23, 2022

相关概念视频

Bacterial Meningitis

Bacterial Meningitis

Bacterial meningitis is a severe infectious disease involving inflammation of the meninges, the protective membranes surrounding the brain and spinal cord. It occurs when pathogenic bacteria cross the blood–brain barrier and enter the cerebrospinal fluid. Common causative organisms include Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae type b, Listeria monocytogenes, and Escherichia coli K1. The exact route of entry varies by pathogen and host condition.Routes of Entry...