Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mucosal-associated invariant T cells recognize a tumor-derived metabolite in the DNA synthesis pathway.

Frontiers in immunology·2026
Same author

The circadian clock component BMAL1 enhances macrophage inflammation by nuclear translocation of peroxisomal β-oxidation enzyme MFP2.

Cell reports·2026
Same author

PLA2G12A-driven extracellular vesicle-lipid signaling amplifies pathogenic T cell responses in inflammatory diseases.

Cell reports·2026
Same author

Organ-specific proteomic responses to tetrodotoxin administration in the liver and skin of Takifugu rubripes.

Toxicon : official journal of the International Society on Toxinology·2026
Same author

Explosion Risk of Cardiac Implantable Electronic Devices During Cremation: Experimental and Survey Findings From Japan.

Journal of arrhythmia·2026
Same author

Characterizing lipoprotein profiles in coronary atherosclerosis development through quantitative lipidomics and proteomics approaches.

NPJ cardiovascular health·2026

相关实验视频

Updated: Jul 19, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K

在脂管学方法中评估分离性能和量化准确性.

Noriyuki Tomiyasu1, Yoshihiro Izumi2, Omidreza Heraviadeh1

  • 1Department of Systems Life Sciences, Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan.

Journal of chromatography. A
|July 3, 2025
PubMed
概括

这项研究比较了四种脂管学分析方法:流量注射 (FI),逆相液态染色学 (RP-LC),水友相互作用液态染色学 (HILIC) 和超临界流体染色学 (SFC). 在脂质分析方面,SFC-MS/MS表现出比HILIC-MS/MS更高的染色学性能.

关键词:
流量注入的注入方法水友互动液体色谱学 液体色谱学利皮多米克 (Lipidomics) 是一种消化剂.质谱测量质量谱测量量化准确性 量化准确性 量化准确性逆相液态染色学 逆相液态染色学超临界流体色谱学 超临界流体色谱学

更多相关视频

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.6K

相关实验视频

Last Updated: Jul 19, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.2K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.3K
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.6K

科学领域:

  • 分析化学 分析化学
  • 生物化学 生物化学
  • 质谱测量质量谱测量

背景情况:

  • 脂管学依赖于质谱学,但不同的分析方法会导致结果的变化.
  • 主要方法包括流量注射 (FI),逆相液态染色学 (RP-LC) 和水友相互作用液态染色学 (HILIC).
  • 超临界流体染色学 (SFC) 对分离脂质异构体有希望,但比较研究很少.

研究的目的:

  • 量化比较FI,RP-LC,HILIC和SFC与MS/MS相结合的性能.
  • 为了评估HILIC-MS/MS和SFC-MS/MS之间的染色学性能差异.
  • 根据具体要求,提供关于选择适当的脂管学分析方法的指导.

主要方法:

  • 使用NIST SRM1950等离子体对14个类别的355种脂质物种进行定量分析.
  • 四种方法 (FI,RP-LC,HILIC,SFC) 与三重四极MS/MS结合在相同的条件下进行了评估.
  • 染色学参数 (分析时间,压力,理论板高度,同位素分离) 用于HILIC-MS/MS和SFC-MS/MS进行了比较.

主要成果:

  • 在四种方法中,在六个脂类中没有观察到显著的定量差异.
  • 在其他脂类中发现了显著的量化方法特异性变化.
  • 在染色学参数,包括同位素分离方面,SFC-MS/MS显著优于HILIC-MS/MS.

结论:

  • 所有评估的方法都适用于脂管学,但性能因脂类而异.
  • 与HILIC-MS/MS相比,SFC-MS/MS提供了优越的染色学分离,特别是在同位素方面.
  • 脂管学中的方法选择应与特定的分析需求保持一致,例如脂标,样本可用性和所需的分析时间.