环境因素影响吉尔牛体外胚胎生产
Lethissia Amorim da Silva Coelho1, Rondineli Pavezzi Barbero2, Marco Roberto Bourg de Mello3
1Programa de Pós-Graduação Em Ciência Animal, Universidade Federal Rural do Rio de Janeiro, 23897-000, Seropédica, RJ, Brazil.
Theriogenology
|July 3, 2025
概括
季节,供体年龄和类别影响吉尔牛体外胚胎生产. 新鲜胚胎移植的怀孕率高于玻璃化胚胎,这表明玻璃化减少了该品种的胚胎存活率.
科学领域:
- 动物科学动物科学
- 生殖生物学 生殖生物学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 优化体外胚胎生产 (IVEP) 对于牲畜的遗传进步至关重要.
- 了解影响吉尔乳品品种IVEP成功的因素对于商业胚胎移植计划至关重要.
研究的目的:
- 在巴西商业胚胎移植计划中,评估影响吉尔乳牛体外胚胎生产的因素.
- 评估季节,供体年龄,供体类别 (母牛与母牛) 以及胚胎保存方法 (新鲜与玻璃化) 对IVEP关键参数的影响.
主要方法:
- 分析了3682名吉尔乳品捐赠者的15894个卵泡愿望数据.
- 评估每个捐赠者的可活性卵子细胞 (VON),可活性卵子细胞比例 (VOP),胚胎细胞率 (BLR) 和怀孕率 (PR).
- 在不同季节,年龄类别,捐赠者类别和胚胎治疗中对IVEP参数进行统计比较.
主要成果:
- 在夏季和春季观察到更高的活力卵细胞比例 (VOP);在春季观察到更高的胚胎细胞率 (BLR).
- 在6-12岁的捐赠者中,最佳VOP;在4岁以上的捐赠者中,和牛相比牛犊中,BLR更高.
- 新鲜胚胎移植 (41.1%) 与玻璃化胚胎 (34.6%) 的妊娠率 (PR) 更高;新鲜胚胎移植的PR在春季和冬季最高.
结论:
- 季节,供体年龄和供体类别显著影响吉尔牛体外胚胎生产结果.
- 玻璃化对体外生产的吉尔乳制品胚胎的生存和发育潜力产生负面影响.
- 这些发现为优化吉尔牛胚胎转移计划的管理和提高效率提供了宝贵的见解.
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