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相关概念视频

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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pre-mRNA Processing02:01

pre-mRNA Processing

53.6K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

10.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
10.0K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.2K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.2K
Experimental RNAi02:15

Experimental RNAi

6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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相关实验视频

Updated: Sep 17, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

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在前体microRNA-20a内部的结构特征 调节Dicer-TRBP处理

Yaping Liu1, Cade T Harkner2, Megan N Westwood1

  • 1Biophysics Program, University of Michigan, 930 N. University Avenue, Ann Arbor, MI 48109, USA.

Journal of molecular biology
|July 3, 2025
PubMed
概括
此摘要是机器生成的。

微RNA (miRNA) 处理由像环和特定核酸突起等结构元素调节. 这些特征控制酶处理,对于适当的基因表达调节至关重要.

关键词:
这就是说,Dicer.核磁共振光谱法 (NMR) 是一种光谱法.结构 RNA 结构 RNA 结构萨克斯 (SAXS) 的时间这是一个微型RNA.

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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相关实验视频

Last Updated: Sep 17, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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MicroRNA-based Regulation of Picornavirus Tropism
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MicroRNA-based Regulation of Picornavirus Tropism

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科学领域:

  • 分子生物学分子生物学
  • 结构生物学 结构生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 微RNA (miRNA) 是小型非编码RNA,在转录后调节基因表达.
  • 精确控制miRNA生物生成对于维持细胞平衡至关重要.
  • 控制miRNA前体处理的分子机制尚不完全理解.

研究的目的:

  • 阐明前miR-20a处理的结构决定因素.
  • 了解结构特征如何调节致癌性miRNA的酶成熟.

主要方法:

  • 使用核磁共振 (NMR) 光谱测定预-miR-20a的溶液结构.
  • 小角度X射线散射 (SAXS) 分析.
  • 评估miR-20a前的处理效率.

主要成果:

  • 在miR-20a前确定了一个灵活的尖端环和一个单核酸突起在Dicer-TRBP裂变部位附近.
  • 证明了替代的顶端环形状可以自我调节Dicer-TRBP处理.
  • 表明在-5位置的凸起对于高效的miR-20a前处理至关重要.
  • 发现与疾病相关的单核酸多态性损害了因结构破坏而导致miR-20a前处理.

结论:

  • 在pre-miR-20a的尖端环中的过渡性RNA构造自我调节其成熟.
  • 特定的结构特征,包括核酸膨胀,对于高效的miRNA处理至关重要.
  • 对miR-20a前的结构洞察力为了解oncomiR-1集群调节和与疾病相关的突变提供了基础.