协生微生物的开发和表征,以提高益生菌在食品应用中的活力
Hammad Naeem1, Muhammad Shahbaz1, Umar Farooq1
1Department of Food Science and Technology, Faculty of Food and Home Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, Pakistan.
Journal of food science
|July 3, 2025
概括
这项研究开发了一种新的共生微珠配方,使用藻酸盐 (Na-Alg),乳清蛋白分离物 (WPI) 和果糖-寡糖化物 (FOS) 来增强Lactobacillus rhamnosus GG的活力. 封装在模拟的胃肠道条件下显著改善了益生菌的存活率,用于食品应用.
科学领域:
- 生物聚合物科学 生物聚合物科学
- 食品微生物学 食品微生物学
- 益生菌技术 益生菌技术
背景情况:
- 益生菌需要有效的输送系统才能在胃肠道条件下生存.
- 藻酸盐 (Na-Alg),乳清蛋白分离物 (WPI) 和果糖-寡糖化物 (FOS) 是适合封装的生物聚合物.
- 拉科巴西勒斯 (Lactobacillus rhamnosus GG) 是一种有益的益生菌菌株,有可能用于食品应用.
研究的目的:
- 开发和描述封装Lactobacillus rhamnosus GG. GG. 的新型共生微粒.
- 在模拟的胃肠道条件下评估封装益生菌的稳定性和活力.
- 评估Na-Alg/WPI/FOS矩阵在食品系统中增强益生菌传递的潜力.
主要方法:
- 使用Na-Alg,WPI和FOS的挤出方法封装Lactobacillus rhamnosus GG. 这是一个非常好的方法.
- 微珠的直径,封装效率,形态 (SEM),分子相互作用 (FTIR) 和热稳定性 (TGA) 的表征.
- 在模拟的胃肠道条件下对封装益生菌活力的体外评估.
主要成果:
- 微粒的平均直径为1.13毫米,封装效率为93%.
- SEM证实了微粒中成功加载益生菌.
- 在FTIR分析中,由于封装材料之间强烈的结合,显示出稳定的矩阵配方.
- 封装的益生菌在模拟的胃肠道条件下显著增强活力.
结论:
- 新的Na-Alg/WPI/FOS复合微粒为封装Lactobacillus rhamnosus G.G.提供了一个稳定的矩阵.
- 这种协生性封装策略显著提高了益生菌的稳定性和生存率.
- 开发的微粒显示出改善益生菌在食品和系统中的传递的巨大潜力.
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