相关实验视频

Updated: Sep 17, 2025

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Published on: October 19, 2017

在感染期间,巨细胞捕获活中性粒细胞

Kelley N Cooper¹, Marina Terekhova², Barbara Potempa³

¹Center for Microbe and Immunity Research, Nationwide Children's Hospital, Columbus, OH, USA.

Cell death & disease

|July 3, 2025

概括

Porphyromonas gingivalis (Pg) 感染导致活中性粒细胞被巨细胞捕获,绕过正常的免疫反应. 这种新的免疫颠覆促进炎症和细菌的持久性.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
细胞生物学细胞生物学

背景情况:

中性粒细胞对于口腔免疫平衡至关重要,需要平衡的激活和清除.
功能失调的中性粒细胞清除有助于慢性炎症状况,如牙周炎.

研究的目的:

调查Porphyromonas gingivalis (Pg) 与中性粒细胞和巨细胞相互作用的机制.
阐明Pg.使用的一种新型免疫规避策略.

主要方法:

基于N-terminomics的蛋白质定型,以确定中性粒细胞蛋白上的RgpB裂解点.
在捕获活中性粒细胞时对巨细胞的表型和转录重编程的分析.
在体外和体内研究,以评估炎症结果和细菌持久性.

主要成果:

Pg感染或其蛋白酶RgpB通过颗粒蛋白的表面转移诱导活中性粒细胞上的新表位生成.
这些新表位细胞激活巨细胞αMβ2整体蛋白,导致非亡性中性粒细胞的内化.
巨细胞捕获活中性粒细胞会导致炎症结果和受损的分离途径,促进细菌的持久性.

结论:

Pg采用一种独特的免疫颠覆策略,通过诱导活中性粒细胞被分离到巨细胞中.
这一过程绕过已知的细胞路径,阻碍了炎症的解决,促进了细菌的持久性.

更多相关视频

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Published on: November 1, 2019

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Published on: February 24, 2010

相关概念视频

Immune Surveillance by NK Cells and Phagocytes

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

Inflammation

Inflammation