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相关概念视频

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

713
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
713
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

Updated: Sep 17, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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阵列检测使简单而强大的MINFLUX具有很大的定位范围.

Eli Slenders1, Sanket Patil1,2, Marcus Oliver Held1

  • 1Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.

Light, science & applications
|July 3, 2025
PubMed
概括
此摘要是机器生成的。

这项研究介绍了ISM-FLUX,这是一种单分子局部化显微镜 (SMLM) 的新方法. ISM-FLUX简化了MINFLUX (多个指令,多个数据) 的本地化,提高了其范围和易用性.

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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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相关实验视频

Last Updated: Sep 17, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
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Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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科学领域:

  • 超高分辨率显微镜的使用方法
  • 纳米尺度成像成像技术
  • 生物物理学的生物物理.

背景情况:

  • MINFLUX (最小信息,最大分辨率) 提高了单分子局部化显微镜 (SMLM) 超出光子计数限制.
  • 传统的MINFLUX使用代扫描,这可能是复杂的,容易出现错误.

研究的目的:

  • 引入ISM-FLUX,一种使用图像扫描显微镜 (ISM) 的新型MINFLUX实现.
  • 为了提高MINFLUX本地化精度,范围和系统简单性.

主要方法:

  • 通过将ISM与单光子雪崩二极管阵列探测器集成,开发了ISM-FLUX.
  • 利用扫描策略,在轨道内提供精确的定位,在轨道外提供常规的不确定性.

主要成果:

  • 与标准MINFLUX相比,ISM-FLUX实现了强大的本地化,具有更大的有效本地化范围.
  • 新方法简化了MINFLUX架构,减少了系统的复杂性.

结论:

  • ISM-FLUX为MINFLUX超分辨率显微镜提供了一种更强大,更简单的方法.
  • 这一进步有可能提高MINFLUX技术的可访问性和采用性.