染色体层次的基因组组合和注释的斯皮尼巴布斯 caldwellililili
Ziyan Fan1, Wen Lei1, Yuan Fang2
1School of Life Sciences, Nanchang University, Nanchang, 330031, China.
Scientific data
|July 3, 2025
概括
我们介绍了第一个染色体层次的基因组组合,用于水产养殖的关键淡水鱼Spinibarbus caldwelli. 这种高质量的基因组资源将促进这一重要的鱼类物种的遗传育种和保护工作.
科学领域:
- 基因组学就是基因组学.
- 水产养殖是水产养殖的一种方式.
- 鱼类学 鱼类学 鱼类学
背景情况:
- 斯皮尼巴布斯 (Spinibarbus caldwelli) 是一个经济上重要的淡水鱼,属于鱼家族,对中国南部的水产养殖至关重要.
- 由于缺少基因组数据,S. caldwelli.的遗传改进和保护策略受到限制.
研究的目的:
- 为了生成一个高质量的,染色体水平的 Spinibarbus caldwelli 的基因组组合.
- 为S. caldwelli提供必要的基因组资源,以支持水产养殖和保护.
主要方法:
- 使用PacBio HiFi长读,Illumina短读和Hi-C测序进行基因组组.
- 使用 Hi-C 脚手架将结合物在染色体水平结构上.
- 通过BUSCO分析评估基因组的完整性.
主要成果:
- 实现了1.77 Gb的染色体级基因组组装,N50的支架为35.29 Mb.
- 99.14%的结合体被固定在50个染色体上,显示出高度的结构完整性.
- 组装显示了98.27%的BUSCO完整性,包含51,505个预测基因,具有90.83%的功能注释.
结论:
- 染色体水平的基因组组合为Spinibarbus caldwelli提供了一个基本的资源.
- 这种基因组促进了对Cyprinid族群的研究,基因改进计划和S. caldwelli.的保护计划.
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