对采样和样品制备方法的评估,以确定叶片样本中的度和稳定的同位素
Saeed Waqar Ali1,2, Dominik Božič1, Sreekanth Vijayakumaran Nair1,2
1Department of Environmental Sciences, Jožef Stefan Institute, 1000, Ljubljana, Slovenia.
Environmental monitoring and assessment
|July 3, 2025
概括
叶片 (Hg) 的吸收是至关重要的,但方法各不相同. 干燥叶子不会影响Hg水平,但冲洗可以去除Hg,特别是在受污染的区域,影响同位素分析.
科学领域:
- 环境化学环境化学
- 植物生态学植物生态学
- 水银生物地质化学 水银生物地质化学
背景情况:
- 大气中的 (Hg) 的叶子吸收是陆地生态系统的重要沉积途径.
- 不一致的采样和预处理方法使叶子Hg数据和同位素测量的比较变得复杂.
- 这种变异性可以在理解不同环境区间的Hg交换中引入偏见.
研究的目的:
- 评估不同样品干燥和冲洗技术对叶子Hg度和同位素特征的影响.
- 评估这些方法如何影响不同环境和树冠位置的可比性.
- 为树叶中标准化Hg分析提供建议.
主要方法:
- 从城市 (卢布尔雅纳) 和历史污染的 (伊德里亚) 地点收集了欧洲角梁 (Carpinus Betulus L.) 的叶片样本.
- 样品在使用不同的干燥方法 (空气干燥,烤箱干燥) 和冲洗程序 (冲洗,不冲洗) 后进行分析.
- 测量了的度和稳定的同位素比率 (δ202Hg).
主要成果:
- 垂直冠状分层显著影响了Hg度,内部树冠叶表显示了更高的水平.
- 干燥方法对各位点的叶子Hg度没有显著影响 (p > 0.05).
- 冲洗从污染部位的样本中去除了24-60%的Hg (p < 0.01),改变了同位素组成 (δ202Hg从-1.9‰转移到-3.2‰).
结论:
- 干燥方法适合在低环境中进行交叉研究的比较.
- 对于专注于内部植物Hg循环的研究,建议冲洗,但在需要总表面和内部Hg时应避免.
- 标准化样品预处理对于在陆地生态系统中准确评估Hg至关重要.
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