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Chengxue Wang1, Qian Wang2, Wenting Yuan1
1Department of Clinical Laboratory, General Hospital of Tianjin Medical University, Tianjin, 300352, China.
Analytical and bioanalytical chemistry
|July 3, 2025
概括
乙酸乙烯 (EA) 为尿液自由皮质醇 (UFC) 检测提供了比二甲 (DCM) 更安全,更有效的替代品. 这项研究验证了EA的有效性.
科学领域:
- 内分泌诊断的内分泌诊断方法
- 临床化学 临床化学
- 分析毒理学分析毒理学
背景情况:
- 二甲 (DCM) 是一种危险的传统提取剂,用于24小时尿液自由皮质醇 (UFC) 测试.
- 与DCM相关的职业危险需要在实验室诊断中提供更安全的替代方案.
研究的目的:
- 评价乙烯 (EA) 作为UFC测试的新型,更安全的提取剂.
- 评估优化EA配方 (EEG) 的提取效率和稳定性.
- 使用EEG方法确定UFC的生物参考间隔.
主要方法:
- 在乙烯酸乙烯 (EA) 和二甲 (DCM) 之间进行比较的提取效率分析.
- 开发一种稳定EA配方 (EEG) 与抗水解和降低挥发力的剂.
- 对EEG配方的恢复和稳定性测试.
- 在EEG提取后使用化学发光免疫试验 (CLIA) 和液体染色学-并联质谱法 (LC-MS/MS) 确定生物参考间隔.
主要成果:
- 与DCM相比,EA表现出明显更高的提取效率和中位数皮质醇水平 (p=0.002).
- 在EA中,恢复率 (95.3%) 与DCM (65.4%,p=0.011) 相比较高.
- 稳定的EEG配方保持了提取效率,并表现出更好的稳定性.
- 确定的95%的参考间隔为14.58136.62μg/24小时 (CLIA) 和0.8160.55μg/24小时 (LC-MS/MS).
结论:
- 乙酸乙烯提取是一种更安全,更有效的替代品,用于UFC分析的DCM.
- 优化的EEG配方为内分泌诊断提供可靠和稳定的提取.
- 新建立的,方法特定的参考间隔有助于准确的临床UFC评估.
- 这一创新支持在日常实验室实践中替换危险溶剂,而不会影响分析性能.
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