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针对heptad重复和融合:纳米粒子疫苗引起粘膜免疫反应对SARS-CoV-2变种

Chaofeng Liang^1,2,3, Rong Li^3,4, Zeyu Pu³

¹Graduate School of Guangzhou Medical University, Guangzhou, 510000, China.

Journal of nanobiotechnology

|July 3, 2025

概括

一种针对保存的SARS-CoV-2尖端蛋白区域的新型纳米粒子疫苗提供了广泛的保护. 鼻内输送引起了强大的粘膜免疫力,并保护小鼠免受Delta和Omicron变异.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学
病毒学病毒学

背景情况:

新出现的SARS-CoV-2变种需要先进的疫苗策略来提供广泛和持久的保护.
目前的疫苗在提供对各种病毒菌株的全面防御方面面临挑战.

研究的目的:

开发和评估FP-HR5纳米粒子疫苗用于鼻内输送的疗效.
评估疫苗诱导针对SARS-CoV-2变种的全身和粘膜免疫的能力.

主要方法:

开发一种纳米粒子疫苗 (FP-HR5-NP),使用铁素平台,准保存的尖端蛋白子单元 (S2,融合,七度重复).
在HACE2转基因小鼠中进行鼻腔免疫.
评估幽默 (IgG,IgA) 和细胞 (B_RM,T_RM) 免疫反应.
挑战使用Delta和OmicronSARS-CoV-2变种的研究.

主要成果:

鼻内FP-HR5-NP疫苗接种诱导了高标位的特定IgG抗体和高分泌IgA和IgG在支气管支气管洗液.
疫苗接种后观察到肺内存B细胞和T细胞的显著增加.
三次剂量给予了对Delta和Omicron变异的实质性保护,没有可检测的病毒RNA或肺部病理.

结论:

FP-HR5-NP疫苗有效地刺激了综合性粘膜和全身免疫力.
鼻内注射提供了针对SARS-CoV-2变种的广泛保护,表明其作为通用COVID-19疫苗的潜力.

关键词:

广泛的疫苗疫苗.保存的表观体是可以保存的.粘膜免疫力粘膜免疫力纳米粒子疫苗纳米粒子疫苗呼吸系统病毒呼吸系统病毒这就是SARS-CoV-2病毒.

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Conjugated Proteins

Conjugated Proteins

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