来自假肢关节感染的金黄色葡萄球菌和血液培养显示相同的遗传背景
Bo Söderquist1,2,3, Peter Wildeman1,2, Marc Stegger1,4,5
1School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
概括
血源性假肢关节感染 (PJIs) 由于黄金杆菌菌菌经常涉及相同的细菌菌株. 基因组分析揭示了最小的差异,支持血源性播种是PJI的原因.
科学领域:
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
- 整形外科 整形外科 整形外科
背景情况:
- 血源性假肢关节感染 (PJIs) 是一个显著的并发症,特别是在Staphylococcus aureus bacteremia之后.
- 在S. aureus bacteremia之后,患PJI的风险很大 (30% - 40%).
研究的目的:
- 为了确定相同的S. aureus菌株是否会在同一患者中引起细菌病和PJI.
- 分析PJI患者血液和组织中S. aureus分离物之间的基因组变异.
主要方法:
- 全基因组测序是在从血液培养和组织活检中获得的配对黄金色杆菌分离物上进行的.
- 包括患有PJI和并发性S. aureus细菌病的患者 (2005-2020年).
主要成果:
- 分析了23名患者的24次PJI发作.
- 所有配对的分离物共享相同的多部位序列类型,克隆复合体和核心基因组多部位序列类型 (cgMLST) 复合类型.
- 配对分离物之间的核心基因组的中位数差异仅为3个单核酸多态 (SNP).
结论:
- 同样的cgMLST复杂类型和较低的SNP分歧表明出血性播种.
- 这些发现强烈支持血液感染作为PJI的来源在这个队列.
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