人体解剖学的空间地图 (SAHA):跨人类器官的多模式亚细胞分辨率参考
Jiwoon Park1,2, Roberto De Gregorio1,3, Erika Hissong1,4
1Weill Cornell Medicine, New York, NY, USA.
bioRxiv : the preprint server for biology
|July 4, 2025
概括
人体解剖学的空间地图 (SAHA) 是一个新的资源,以亚细胞分辨率绘制人体组织. 这本地图揭示了细胞和相互作用,有助于疾病研究和精准医学.
科学领域:
- 人类解剖学 人类解剖学
- 空间生物学 空间生物学
- 基因组学就是基因组学.
背景情况:
- 了解人类组织组织对于疾病研究至关重要.
- 现有的地图集缺乏多式联络,亚细胞分辨率数据.
- 细胞和组织之间的空间关系是生物功能的关键.
研究的目的:
- 创建第一个健康成年人组织的多式,亚细胞分辨率的参考地图.
- 在多个器官系统中绘制保存和器官特定的细胞.
- 为空间诊断和精准医学提供一个基础框架.
主要方法:
- 空间转录组学,蛋白质组学和组织学在>1500万个来自>100个捐赠者的细胞中进行整合.
- 使用CosMx SMI,10x Xenium,RNAscope,GeoMx DSP和单核RNA-seq. 的高分辨率分析.
- 与结直肠癌和炎症性肠病数据集进行比较分析.
主要成果:
- 在胃肠道和免疫组织中详细地绘制细胞.
- 识别空间组织的细胞状态和罕见的适应性免疫群体.
- 检测与疾病相关的空间干扰,包括密码脱差和免疫重塑.
结论:
- 人体解剖学的空间地图 (SAHA) 为研究人类组织组织提供了前所未有的资源.
- 萨哈可以检测与癌症和IBD等疾病相关的空间干扰.
- 开放式可访问的数据支持探索,基准测试和机器学习,以推进精准医学.
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