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在Fusobacterium nucleatum和糖捕食者Segatella oris之间的交叉食相互作用

Joshua R Fletcher¹, Ryan C Hunter²

¹Department of Population Health and Pathobiology, North Carolina State University College of Veterinary Medicine, Raleigh, NC 27606.

bioRxiv : the preprint server for biology

|July 4, 2025

概括

Fusobacterium nucleatum与Segatella oris的相互作用显示出复杂的交叉养和对抗性. 这些动态影响营养使用,生物膜形成以及呼吸道和瘤环境中的宿主反应.

科学领域:

微生物学微生物学
口腔微生物组是指口腔的微生物组.
主体与微生物的相互作用

背景情况:

Fusobacterium nucleatum是一种常见的口腔细菌,与各种感染和疾病有关.
它在多微生物环境中与其他微生物的相互作用尚未得到充分理解.

研究的目的:

研究Fusobacterium nucleatum和Segatella oris.之间的物种间动态和交叉食相互作用.
了解这些相互作用如何影响营养获取,生物膜形成,基因表达和宿主反应.

主要方法:

利用了培养,无细胞超,以及在人类呼吸道上皮质细胞上共同培养.
进行双重和三重RNA测序 (RNA-seq) 来分析基因表达.
研究了营养获取,生物膜形成和宿主炎症反应.

主要成果:

塞加特拉·奥里斯超剂增强了Fusobacterium nucleatum的生长,并诱导了其酸代谢基因.
Fusobacterium nucleatum调节的Segatella oris多糖利用位点 (PULs) 的表达.
塞加特拉奥里斯抑制了Fusobacterium nucleatum生物膜的形成,表明了对抗性.
共殖民调节宿主炎症和癌症相关的基因表达.

结论:

甘氨酸介导的交叉养和微生物相互作用显著影响了Fusobacterium nucleatum的生理学和病原性.
在与宿主相关的条件下模拟多微生物群落对于理解病原生物的行为至关重要.

关键词:

杆菌核酸 (Fusobacterium nucleatum) 是一种细菌.普雷沃特拉·奥里斯 (Prevotella oris) 是一种的植物.塞加特拉鱼 (Segatella oris) 是一个有趣的动物.慢性呼吸道疾病慢性呼吸道疾病交叉养 - 交叉养是什么?糖甘油的使用方法微生物组是一个微生物组.翻译学翻译学翻译学翻译学

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