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卡普赛西诺酸葡萄糖酸通过TRPV1/AMPK依赖信号通路减轻HepG2细胞中的脂质积累

Abdeen Elkhedir^1,2, Alsadig Yahya³, Eihab H Jadelrab²

¹Agro-Industries Institute, Industrial Research and Consultancy Centre (IRCC) Khartoum Sudan.

Food science & nutrition

|July 4, 2025

概括

卡普赛西诺酸葡萄糖 (CG) 通过减少脂质积累和氧化应激,有效降低肝细胞中的甘油三和胆固醇. 这种新型化合物通过TRPV1/AMPK通路起作用,显示出对代谢健康的潜在影响.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
营养学科学营养学科学

背景情况:

过度脂质积累的代谢障碍是一个主要的全球健康问题.
与素相比,Capsaicinoid glucoside (CG) 是一种水溶性素衍生物,提供了增强的生物利用性和减少的尖性.

研究的目的:

为了研究CG的降脂效应.
阐明CG在肝细胞中的作用的潜在分子机制.

主要方法:

用油酸 (OA) 诱导的HepG2细胞模型来模拟肝脏脂质积累.
甘油三 (TG) 和总胆固醇 (TC) 水平的定量分析.
通过Oil Red O染色测量细胞内活性氧物种 (ROS) 和脂质滴积累.
关键代谢调节者的基因和蛋白质表达分析 (TRPV1,AMPK,SREBP-1c,FASN,PPARδ).
分子对接模拟以评估CG与TRPV1和AMPK的相互作用.

主要成果:

在OA诱导的HepG2细胞中,CG治疗 (100μg/mL) 显著降低了TG60.7%,TC降低了34.4%.
CG显著降低了细胞内ROS水平和脂质滴滴积累.
CG上调TRPV1和AMPK表达,同时降低脂原性标记物 (SREBP-1c,FASN) 和上调脂氧化标记物 (PPARδ).
分子对接证实了CG与TRPV1和AMPK的稳定结合.

结论:

在肝硬化症的细胞模型中,CG显示出显著的降脂和抗氧化特性.
观察到的效应通过TRPV1/AMPK信号通路进行介导.
CG具有作为功能性食品成分或治疗补充剂的潜力,用于控制肝脂代谢.

关键词:

这就是AMPKK.卡普赛奇诺伊德葡萄糖化物在HepG2细胞中.在 TRPV1 中.细胞信号通道的通道.脂质新陈代谢脂质新陈代谢

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