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什么时候最好测试结核病阳性? 来自匈牙利奶牛群的后续研究的观察结果

Barbara Vass-Bognár¹, Mikolt Bakony², Kinga Fornyos³

¹Department of Animal Hygiene, Herd Health and Mobile Clinic, University of Veterinary Medicine, Budapest, Hungary.

Frontiers in veterinary science

|July 4, 2025

概括

早期检测Mycobacterium avium ssp. 这种细菌. 在乳牛中对准结核病 (MAP) 的治疗至关重要. 该研究确定牛奶中40-60天是使用血清ELISA和PCR试验进行MAP查的最佳时期.

科学领域:

兽医微生物学兽医微生物学
动物卫生动物健康
乳制品科学乳制品科学

背景情况:

这种细菌是Mycobacterium avium ssp. 准结核病 (MAP) 导致约翰氏病,这是乳牛群的一个重大问题.
准确及时诊断MAP感染对于有效的群体管理和疾病控制至关重要.

研究的目的:

确定最实用的诊断方法来识别感染MAP的奶牛.
在哺乳期确定最佳时间点,用于诊断样本采集和测试.

主要方法:

在匈牙利四个奶牛场进行了52头MAP阳性孕母牛的纵向研究.
在多个时间点采集便,血液和牛奶样本 (1-300天在牛奶中).
应用ELISA和PCR测定用于抗体和病原体检测.

主要成果:

在患有临床结核病的动物和没有患有临床结核病的动物之间观察到不同的诊断模式.
通过使用血清ELISA,在牛奶中 (DIM) 40-60天内实现了MAP的最佳检测概率.
DIM 10-14和40-60在PCR测定中显示出最佳结果,血清ELISA显示出比牛奶ELISA稍高的灵敏度.

结论:

在牛奶中40-60天的时间段是最适合对乳牛进行MAP感染查的.
结合诊断程序和战略时间点,提高了在哺乳期早期识别受感染动物的可能性.

关键词:

艾丽莎·伊丽莎·伊丽莎奶牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛有关结核病的结核病.qPCRR 是一个很好的方法.采样时间采样时间

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