相关实验视频

Updated: Sep 17, 2025

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

TFAP4通过调节机械传导来加剧病态心脏纤维化

Jie Liu^1,2, Jingjing Feng³, Jingxuan Zhao^1,2

¹Department of Cardiology, Zhongnan Hospital of Wuhan University, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan 430071, Hubei, China.

|July 4, 2025

概括

转录因子TFAP4通过激活机械传感器Itga11和Piezo2.2,驱动心脏纤维化. 向TFAP4可能会减少纤维化,并改善心肌梗塞 (MI) 后的心脏功能.

科学领域:

心脏病学心脏病学
分子生物学分子生物学
生物医学工程生物医学工程

背景情况:

心脏纤维细胞 (CF) 分化为肌纤维细胞驱动心脏纤维化和心肌硬化.
机械传导是CF激活和肌肉发作 (MI) 后纤维化的一个关键调节器.
关联CF机械线索与激活的分子机制尚不清楚.

研究的目的:

确定CF激活和纤维化的分子调节剂.
研究转录因子TFAP4在心脏纤维化中的作用.
探索TFAP4与机械传导通路的联系.

主要方法:

利用人类和小鼠心脏纤维细胞模型.
过度表达和沉默的TFAP4,Itga11和Piezo2. 这两种类型.
评估CF的扩散,分化和细胞外基质 (ECM) 沉积.
在MI后体内评估心脏功能和纤维化.

主要成果:

过度表达TFAP4增强了CF扩散,ECM产生和肌纤维细胞分化.
TFAP4直接激活了机械传感器基因Itga11和Piezo2.
沉默Itaga11/Piezo2扭转了TFAP4诱导的亲纤维化作用.
降低TFAP4调节减少了纤维化和心脏功能改善后MI.

结论:

TFAP4是心脏纤维化的一个中央调节器,将机械传导与纤维化联系起来.
TFAP4 直接控制关键机械传感器 Itga11 和 Piezo2.
TFAP4代表了缓解心脏纤维化的潜在治疗标,并增强后心脏冲击的恢复.

关键词:

心脏纤维化是什么心脏纤维化纤维细胞激活的激活机械转导是指机械转导的过程.在TFAP4中,我们可以使用TFAP4.

更多相关视频

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Published on: June 14, 2016

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

相关概念视频

Heart Failure II: Pathophysiology

Heart Failure II: Pathophysiology

Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...

Cell-matrix's Response to Mechanical Forces

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...