乳酸盐通过基因素乳化修饰调节红色素原体细胞的细胞分化
Qianqian Yang1, Hengchao Zhang1, Yan Hou1
1School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China.
iScience
|July 4, 2025
概括
乳酸盐通过调节组织素乳酸化来影响红细胞的产生. 这项研究揭示了乳酸水平如何通过表观遗传修饰影响早期红色素原始细胞分化和基因表达.
科学领域:
- 生物化学 生物化学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 血液学 血液学 血液学
背景情况:
- 乳酸盐是一种关键的代谢物,参与细胞能量和通过基因素乳化进行表观遗传调节.
- 乳酸和组织素乳化在人类红细胞发育 (红细胞形成) 中的具体作用尚不清楚.
研究的目的:
- 为了研究乳酸盐水平对人体红色素质的影响.
- 为了探索乳酸,基因素乳化和红色素原体细胞分化和基因表达的调节之间的关系.
主要方法:
- 使用2-脱氧-d-葡萄糖 (2-DG) 操纵细胞内乳酸盐水平以抑制糖解或添加外源的乳酸 (Na-La).
- 评估红色素原体的分化,细胞循环的进展和殖民地形成单位-红色素 (CFU-E) 的形成.
- 分析基因素乳化 (H3K14la) 水平和细胞周期和分化相关基因的表达.
主要成果:
- 抑制糖解促进了红色素原体的分化和细胞循环停止,同时减少了CFU-E的形成.
- 升高的乳酸盐水平延迟了红色素原体的分化,并增强了CFU-E殖民地形成.
- 发现乳酸盐水平直接调节H3K14la强度,影响细胞周期和多能性相关的关键基因的表达.
结论:
- 乳酸在调节早期红色素原生细胞分化方面发挥着重要作用.
- 基因素乳化是乳酸通过影响基因表达和细胞过程在红细胞生成过程中的关键机制.
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