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基因组和转录组广泛关联研究确定了在玉米中调节叶酸水平的候选基因
Chenglin Zou1, Meng Yang1, Aihua Huang1
1Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, China.
Frontiers in plant science
|July 4, 2025
概括
这项研究揭示了控制玉米中叶酸水平的关键基因,这些基因使用多omics. 这些发现为开发具有更高营养价值的玉米铺平了道路.
科学领域:
- 植物遗传学 植物遗传学
- 营养生物化学 营养生物化学
- 生物信息学是一种生物信息学.
背景情况:
- 玉米 (Zea mays L.) 是一种重要的食叶酸来源.
- 影响玉米天然叶酸变化的遗传因素尚不清楚.
研究的目的:
- 阐明控制玉米中叶酸积累的分子机制.
- 确定调节玉米核中的叶酸含量的关键基因.
主要方法:
- 综合的多omics方法包括RNA-seq,GWAS和TWAS.
- 使用HPLC量化叶酸含量和确定差异表达基因 (DEGs).
- 使用qRT-PCR验证了候选基因表达.
主要成果:
- 鉴定了137种富含烯胺生物合成和应激反应途径的DEG.
- GWAS确定了2,153个候选基因;综合分析确定了7个关键基因.
- TWAS确定了13个参与叶酸生物合成的因果基因,在高叶酸玉米中验证了更高的表达.
结论:
- 确定了影响玉米叶酸积累的关键调节基因.
- 为开发具有增强营养价值的生物强化玉米提供了见解.
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