WNT5B通过影响mRNA稳定性来调节肌体发生和纤维类型转换
Danyang Fan1,2,3, Yilong Yao1,2,3, Chao Yan1,2,3
1Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
International journal of biological sciences
|July 4, 2025
概括
通过增强细胞增殖和肌纤维发育,WNT5B促进骨肌肉的生长. 在WNT5B中有一个特定的SNP.
科学领域:
- 分子生物学分子生物学
- 发育生物学 发展生物学
- 动物遗传学动物遗传学
背景情况:
- 无翼集成 (WNT) 信号通路对于肌肉形成至关重要.
- 对于WNT5B在骨肌肉发育中的特定作用和调节机制尚不完全理解.
研究的目的:
- 为了阐明WNT5B在猪骨髓生成中的分子机制.
- 研究WNT5B单核酸多态 (SNP) 对肌肉发育的影响.
主要方法:
- 在肥胖 (Tongcheng) 和瘦 (Landrace) 猪中对WNT5B表达的比较分析.
- 在体外研究骨肌细胞增殖,细胞亡和细胞循环.
- 在体内评估肌纤维特性和肌肉纤维类型.
- 通过AU丰富元素 (ARE) 和微RNA相互作用 (miR-29a/b/c) 对WNT5BmRNA稳定性的SNP影响的分析.
主要成果:
- WNT5B在猪骨肌肉中表达很高,并影响肌肉发生.
- 在体外,WNT5B促进细胞增殖和细胞循环,同时抑制细胞亡.
- 在体内,WNT5B增强肌纤维厚度,增加慢型肌纤维.
- 在WNT5B 3'UTR中的SNP (c.1608 A > G) 通过调节miR-29a/b/c相互作用来影响mRNA稳定性.
结论:
- WNT5B在调节肌肉发生过程中起着至关重要的作用.
- 确定的SNP影响WNT5BmRNA的稳定性,影响骨肌肉的发育.
- WNT5B是一种潜在的候选基因,可以改善猪的繁殖特征.
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