在伊朗马什哈德从移民患者中采集的Mycobacterium tuberculosis菌株的基因型多样性,采用MIRU-VNTR方法
Mahbubeh Jangi1, Kiarash Ghazvini2, Saman Soleimanpour2
1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Iranian journal of microbiology
|July 4, 2025
概括
在马什哈德的移民患者中,Mycobacterium结核病菌株的遗传多样性揭示了各种基因型,包括NEW-1和CAS/Delhi. 鉴定出了少量的多药耐药菌株,突出显示了移民查的必要性.
科学领域:
- * 传染病的分子流行病学.
- * 细菌病原体的基因组分析.
背景情况:
- *结核病 (TB) 仍然是一个全球性的健康挑战,移民人口经常面临独特的风险.
- *了解Mycobacterium结核病 (Mtb) 的遗传多样性和耐药性模式对于有效的控制策略至关重要.
研究的目的:
- * 调查来自马什哈德移民患者的Mtb菌株的遗传多样性和家族遗传关系.
- * 评估这些mtb分离物的药物敏感性概况,特别是针对利法和异化.
主要方法:
- *在52个唾液样本上进行了菌间隔重复单元可变数并列重复 (MIRU-VNTR) 排型.
- * 用比例方法进行了对利法 (RIF) 和异化 (INH) 的药物敏感性测试.
- * 采用Xpert MTB/RIF和多重等位基因特异性PCR (MAS-PCR) 来进一步确定耐药性.
主要成果:
- *对52个Mtb分离物的分析显示出显著的遗传多样性,其中NEW-1 (34.6%) 和CAS/Delhi (32.7%) 是最常见的基因型.
- *在3.8%的RIF分离物中观察到药物耐药性,一个分离物对RIF和INH都表现出多重耐药性 (MDR).
- * 在研究组中,近期结核病传播的估计率为0.21%.
结论:
- * 在阿富汗移民中发现的多样化的Mtb基因型可能有助于结核病的重新激活.
- *耐药菌株的存在强调了在边境对移民进行查的重要性.
- *有效的结核病控制需要针对确诊病例制定强有力的治疗和后续方案.
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