相关实验视频

Updated: Jun 20, 2026

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic

Published on: September 4, 2012

在产后奶牛中,子宫内膜巨细胞与子宫内膜炎的持续性之间的关联

C Anastácio¹, G Pereira¹, E Silva¹

¹CIISA- Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Lisbon, Portugal.

Animal : an international journal of animal bioscience

|July 4, 2025

概括

牛产后子宫内膜炎的早期诊断是可能通过分析子宫内膜巨细胞 (Mφ). 产后21天的巨细胞概况可以预测康复或持续的病例,帮助抗菌素耐药性的努力.

科学领域:

兽医医学兽医医学兽医医学
免疫学免疫学免疫学
生殖生物学生殖生物学

背景情况:

产后子宫内膜炎在奶牛中很常见.
发生自发恢复,但需要早期诊断来指导治疗和限制抗菌素耐药性.
子宫内膜巨细胞 (Mφ) 在子宫健康和疾病解决方面发挥作用.

研究的目的:

在产后子宫内膜炎状态方面,描述子宫内膜Mφ的存在,比例和表型.
评估Mφ计数和比率作为预测牛子宫内膜炎恢复或持续性的潜在早期诊断标记.

主要方法:

在产后21天和42天 (DPP) 的173头奶牛的子宫样本中对中性粒细胞 (PMN) 和Mφ的细胞学评估.
对子宫内膜组织段进行免疫标记,以量化M1 (CD86+) 和M2 (CD163+) Mφ表型.
在康复的奶牛和患有持续性子宫内膜炎的奶牛之间比较Mφ档案.

主要成果:

在21 DPP时,晚些时候康复的奶牛比持久病例有更高的Mφ比例和更低的PMN:Mφ比例.
恢复奶牛的M2 Mφ数量较高,M1:M2比率较低,在21 DPP.
持久性子宫内膜炎与42 DPP的总Mφ和M2数量较高有关,这表明Mφ驱动的分辨率受损.

结论:

子宫内膜Mφ形状,特别是在21 DPP时,可以潜在地预测乳牛产后子宫内膜炎的结果.
子宫内膜炎的持续性与Mφ驱动的前置解决机制,特别是M2Mφ功能中的故障有关.
需要进一步的验证研究来证实子宫内膜Mφ在治疗产后子宫内膜炎的诊断效用.

关键词:

牛类牛类牛类牛类牛类牛在子宫内膜炎中,子宫内膜炎.巨细胞是一个巨细胞.中性粒细胞中性粒细胞.这就是Puerperium.

更多相关视频

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

Published on: July 21, 2016

Author Spotlight: Advancing Reproductive Immunology with a Protocol for the Quantitative Evaluation of Endometrial Immune Cells

Author Spotlight: Advancing Reproductive Immunology with a Protocol for the Quantitative Evaluation of Endometrial Immune Cells

Published on: October 13, 2023

相关概念视频

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...

Development of Human Microbiota

Development of Human Microbiota

The human microbiota begins developing at birth and undergoes continual change as we age. Infancy marks a critical period of microbial sensitivity, offering a “window of opportunity” during which beneficial microbes help mature the immune system. By age three, children typically develop a more stable and diverse microbial community. Newborns acquire microbes from their immediate environment; vaginal delivery favors maternal vaginal microbes, while cesarean births favor microbes from the skin...

Development of the Oral Microbiota

Development of the Oral Microbiota

The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...

Chronic Inflammation: Introduction

Chronic Inflammation: Introduction

Chronic inflammation is a prolonged, dysregulated immune response that persists for weeks to years when the inciting stimulus is difficult to eradicate or when self‑antigens drive ongoing reactivity. Morphologically, it is defined by mononuclear cell infiltration, progressive tissue destruction, and concurrent attempts at healing via angiogenesis and fibrosis. Compared with acute inflammation, edema is less prominent while cellular infiltration predominates; triggers include persistent...

Inflammatory Bowel Disease III: Crohn's Disease

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...

Chronic Pancreatitis II: Pathophysiology

Chronic Pancreatitis II: Pathophysiology

Chronic pancreatitis is a progressive and irreversible inflammation of the pancreas, most often caused by long-term alcohol abuse, but it can also be related to ductal obstruction, smoking, or genetic factors.Chronic pancreatitis occurs when the pancreas is repeatedly exposed to harmful agents like alcohol, smoking, ductal obstruction, or genetic predisposition. These factors lead to the release of toxic metabolites and inflammatory cytokines, sustaining chronic inflammation in the pancreatic...