红树林相关的耐性细菌增加了Solanum lycopersicum中的盐度应激耐受性
Mohamed AlHosani1, Sajeesh Kappachery1, Tanveer Alam Khan1
1Biology Department, College of Science, United Arab Emirates University, P.O. Box 15551, Al-Ain, United Arab Emirates.
概括
来自红树林生态系统的植物生长促进根茎细菌 (PGPR) 提高了番茄盐的耐受性. 细菌细菌和细菌西安菌在盐水条件下显著提高了植物的生长和抗压能力.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 环境科学 环境科学
背景情况:
- 度压力对全球的作物产量产生了负面影响.
- 促进植物生长的根茎细菌 (PGPRs) 提供了一种可持续的解决方案,以减轻非生物压力.
- 红树林生态系统是具有有益特征的耐性PGPRs的丰富来源.
研究的目的:
- 从阿布扎比的高盐度红树林生态系统中分离和描述PGPRs.
- 评估选择的PGPRs在增强番茄植物盐应激耐受性的有效性.
- 为了确定特定的PGPR特征,如ACC除氨酶活性,与改善植物性能相关.
主要方法:
- 从红树林根层中分离和选耐盐细菌,以检测ACC除氨酶 (ACCD) 和酸盐溶解 (P+) 活性.
- 在体外和温室实验中,评估PGPR注射对不同盐度 (高达75毫米NaCl) 的番茄苗木生长的影响.
- 生理学评估包括叶绿素光 (光系统II效率) 和叶子光谱反射率指数.
主要成果:
- 57个耐盐分离物被选;24个显示出积极的植物生长促进 (PGP) 特性.
- 九个分离物增强了75mM NaCl的亚格板上的苗木生长;四个 (B1-B4) 进行了温室试验.
- 在盐水条件下,Bacillus subtilis (B1) 和Bacillus siamensis (B3) 显著改善了番茄生长,产量参数 (芽/根重量,花数) 和生理指标 (PSII效率,光谱指数).
- 细菌 velezensis (B2) 显示中等效益,而 Priestia 丝状 (B4) 的影响最小,与 ACCD 活动和 IAA 生产相关联.
结论:
- 精选的PGPRs,特别是Bacillus subtilis (B1) 和Bacillus siamensis (B3),有效地减轻番茄植物中的盐应激.
- ACCD活动和IAA产生是PGPR的关键特征,有助于增强盐耐受性.
- 这些强大的PGPR菌株显示出作为生物肥料的巨大潜力,用于在盐水环境中可持续生产西红.
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