相关实验视频

Updated: Sep 16, 2025

Murine Model of Thoracic Aortic Dissection Induced by Oral β-Aminopropionitrile and Subcutaneous Angiotensin II Infusion

Murine Model of Thoracic Aortic Dissection Induced by Oral β-Aminopropionitrile and Subcutaneous Angiotensin II Infusion

Published on: May 16, 2025

通过TGF-β/smad信号传导,GALNT4通过调节血管光滑肌细胞表型切换和功能障碍来控制大动脉剖析

Liwei Guo¹, Pengcheng Wei¹, Lulu Zhou¹

¹School of Forensic Medicine, Xinxiang Medical University, Xinxiang, Henan, China; Xinxiang Key Laboratory of Metabolism and Integrative Physiology, Xinxiang, Henan, China.

Biochimica et biophysica acta. Molecular basis of disease

|July 4, 2025

概括

聚酸N-乙烯基银胺基转移酶4 (GALNT4) 在大动脉剖析 (AD) 中升高. 在血管光滑肌细胞 (VSMC) 中减少GALNT4通过调节TGF-β/Smad通路来改善AD病理.

科学领域:

心血管生物学心血管生物学
分子医学是分子医学.
葡萄糖科学 (Glycoscience) 是一种科学.

背景情况:

大动脉解剖 (AD) 是一种严重的血管状况,分子驱动因素不明.
聚酸N-乙 галактозаминанил转移酶4 (GALNT4) 与心血管疾病有关,但其在阿尔茨海默病中的作用尚未确定.

研究的目的:

研究大动脉剖析中的GALNT4的表达和功能.
阐明GALNT4影响AD血管光滑肌肉细胞 (VSMC) 行为的分子机制.

主要方法:

在人类AD组织和小鼠AD模型中分析GALNT4表达.
在小鼠模型和体外实验室中进行了针对VSMC的GALNT4倒置和过度表达研究.
评估AD发病率,破裂率,VSMC标记物和TGF-β/Smad信号通路的激活.

主要成果:

在人类AD患者和AD小鼠的动脉中,GALNT4的表达显著上调.
特定于SMC的GALNT4倒置降低了AD的发生率和破裂,保存了收缩标记物并抑制了合成标记物.
GALNT4敲击抑制了Ang II诱导的VSMC表型切换和迁移,与TGF-βR2的O-GalNAcylation降低和Smad信号抑制有关.

结论:

在AD中,GALNT4在调节VSMC表型切换和功能障碍方面发挥着关键作用.
TGF-β/Smad信号通路由GALNT4依赖的糖化调节,是AD病变发生的关键机制.
对于大动脉剖析干预来说,GALNT4 是一个潜在的治疗点.

关键词:

动脉剖析是一个大动脉剖析.盖伦特4 盖伦特4 在TGF-β/Smad信号传输中.在VSMC的表型切换中.

更多相关视频

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Published on: September 14, 2021

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Published on: February 15, 2022

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...