Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Antibiotic Selection00:57

Antibiotic Selection

55.3K
Overview
55.3K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

219
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
219
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

128
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
128

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

'Comparing serotype coverage of pneumococcal vaccines with PCV21 (V116), a new 21-valent conjugate pneumococcal vaccine, and the epidemiology of its eight unique Streptococcus pneumoniae serotypes (15A, 15C, 16F, 23A, 23B, 24F, 31 and 35B) causing invasive pneumococcal disease in adult patients in Canada: SAVE study, 2018-2021-right-to-reply'.

The Journal of antimicrobial chemotherapy·2026
Same author

Mechanisms of fosfomycin resistance observed in clinical isolates of <i>Escherichia coli</i> from Canada: CANWARD 2007-2022.

JAC-antimicrobial resistance·2026
Same author

Streptococcus pneumoniae upregulates Toll2, Toll9, and defensin genes in Bombyx larvae infection model.

PloS one·2026
Same author

Enterococcus faecium harbouring vanA, optrA and cfr(D) on a linear plasmid in Canada: a CANWARD surveillance case.

The Journal of antimicrobial chemotherapy·2026
Same author

Rethinking the unmet need for novel antibiotics.

The Journal of antibiotics·2026
Same author

Eravacycline: Data Based on Real Evidence.

The new microbiologica·2025

相关实验视频

Updated: Sep 16, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.9K

大规模实验室进化揭示了临床相关的附带抗生素敏感性.

Farhan R Chowdhury1, Veronica Banari2, Vlada Lesnic2

  • 1Department of Biology, Concordia University, Montréal, Québec, Canada.

International journal of antimicrobial agents
|July 4, 2025
PubMed
概括

附带敏感性 (CS) 疗法可以对抗抗生素耐药性. 在大肠杆菌中,tigecycline和polymyxin B之间的新型CS链接在实验室进化方法中强大,并在临床分离物中发现,为抗药性提供了新的策略.

关键词:
适应性实验室进化的演变.抗生素耐药性 抗生素耐药性担保的敏感性 担保的敏感性

更多相关视频

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

9.8K

相关实验视频

Last Updated: Sep 16, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.9K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
10:50

Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection

Published on: September 27, 2016

9.8K

科学领域:

  • 微生物学 微生物学
  • 进化生物学 进化生物学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 抗生素耐药性是一个日益增长的全球健康威胁.
  • 附带敏感性 (CS) 序列疗法是缓解耐药性演变的有希望的策略.
  • CS相互作用的重复性和临床相关性需要进一步研究.

研究的目的:

  • 在不同的实验室适应性进化 (ALE) 条件下评估CS相互作用的进化重复性.
  • 为了确定新的CS关系与潜在的临床应用.
  • 确定不同ALE平台对临床CS效应的预测能力.

主要方法:

  • 使用三种不同的ALE平台,对抗tigecycline (TIG) 和piperacillin (PIP) 的大肠杆菌多个系谱的演化.
  • 产生了超过130个耐药突变,并进行了540个耐药性和CS测量.
  • 分析了超过750个多药耐药 (MDR) 大肠杆菌分离物的临床数据集.

主要成果:

  • 在所有ALE平台上确定了TIG和polymyxin B (POL) 之间的高度可重复的CS关系.
  • 该机制涉及TIG耐药性,导致Lon蛋白酶失活和外聚糖过度产生,导致对POL的过敏.
  • 软基梯度演变 (SAGE) 平台展示了对临床附带效应的最佳预测.

结论:

  • 发现的TIG-POL CS相互作用是强大的,并在不同的进化微环境中得到保护.
  • 这种CS关系存在于临床泌尿病原体MDR大肠杆菌分离物中.
  • 建立了一个用于识别临床相关和强大的CS相互作用的框架,有助于制定打击抗生素耐药性的战略.