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在糖尿病病中尿外体miR-142-3p的表达和诊断值评估

Shengjie Li¹, Chuancai Sun^1,2, Shanshan Zheng¹

¹Department of Nephrology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan, China.

Scientific reports

|July 4, 2025

概括

研究人员确定了泌尿外体miR-142-3p作为糖尿病病 (DN) 的有希望的非侵入性生物标志物. 这种微RNA显示出诊断潜力,与疾病进展相关,为DN诊断提供了新的途径.

科学领域:

生物化学生化学
遗传学是一个遗传学.
腎臟病學 (nephrology) 是一種醫學專業.

背景情况:

糖尿病病 (DN) 是糖尿病的严重并发症,也是末期病的主要原因.
脏活检是诊断的黄金标准,存在风险;当前的尿路标记物如微专蛋白已经限制了早期的预测能力.
对于早期和准确的DN诊断而言,非常需要非侵入性生物标志物.

研究的目的:

在糖尿病和DN中选差异表达的微RNA (miRNAs).
评估已识别的miRNAs作为DN的非侵入性生物标志物的诊断价值.
探索这些miRNAs在DN病变发生中的潜在作用.

主要方法:

使用GEO数据库对差异表达的miRNA进行选.
从糖尿病和DN患者收集临床数据和尿液样本.
提取尿外体,然后进行qRT-PCR进行miRNA表达分析.
接收器操作特征 (ROC) 曲线和斯皮尔曼相关性分析用于诊断价值评估.
进行KEGG通路丰富分析,以了解miRNA参与疾病进展.

主要成果:

与2型糖尿病 (T2DM) 患者相比,DN患者的尿外体miR-142-3p显著上调.
在ROC曲线分析中,DN中的miR-142-3p的诊断性能很好.
miR-142-3p与尿中的白蛋白与肌素比率 (UACR) 和慢性病进展指标呈正相关性.
凯格分析表明miR-142-3p参与脂肪酸代谢和Wnt信号传递等途径.

结论:

尿外体 miR-142-3p 显示出作为糖尿病病的非侵入性诊断生物标志物的显著潜力.
这种miRNA可能成为早期检测和DNA风险分层的宝贵工具.
进一步研究miR-142-3p在DN病原发生中的机制作用是有必要的.

关键词:

生物标志物生物标志物糖尿病脏病糖尿病脏病诊断值是一个诊断值.尿道外分泌体尿道外分泌体在 miR-142-3p 中使用.

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