开发一个共享敏感性基因小组作为慢性阻塞性肺病和心力衰竭的诊断生物标志物
Hailong Li1, Cuncun Huang2, Rong Su2
1Department of General Practice, Luohu Clinical Institute of Shantou University Medical College, Shenzhen, 518000, Guangdong Province, PR China; Department of General Practice, Third Affiliated Hospital of Shenzhen University (Luohu District People's Hospital), Shenzhen, 518000, Guangdong Province, PR China; Department of Internal Medicine, First School of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, Gansu Province, PR China.
这项研究确定了四个关键基因 (SVEP1,MOXD1,SMOC2,GNB3) 在慢性阻塞性肺病 (COPD) 和心力衰竭 (HF) 两种疾病中都受到上调调. 这些基因对伴随性疾病的诊断准确度很高.
科学领域:
- 生物信息学是一种生物信息学.
- 基因组学就是基因组学.
- 伴发病性研究 研究.
背景情况:
- 慢性阻塞性肺病 (COPD) 和心力衰竭 (HF) 经常同时存在,带来复杂的临床挑战.
- 导致COPD-HF并发症的潜在分子机制尚不清楚.
研究的目的:
- 使用综合生物信息学系统地识别与COPD-HF共发病相关的分子特征.
- 发现新的诊断生物标志物,阐明这种并发性疾病的病理生理机制.
主要方法:
- 分析COPD和HF数据集中的差异表达基因 (DEG) 和权重基因共表达网络 (WGCNA) 模块.
- 应用最小绝对收缩和选择操作员 (LASSO) 来识别诊断基因.
- 为评估诊断潜力,开发名录和接收器操作特征 (ROC) 曲线分析.
- 单个样本基因组丰富分析 (ssGSEA) 用于调查免疫系统参与.
主要成果:
- 确定了四个显著上调的共同敏感性基因 (SVEP1,MOXD1,SMOC2,GNB3),它们对COPD和HF都很常见 (P<0.001).
- 这些基因表现出高的诊断准确性 (AUC>0.85),诺米图证实了它们的潜力.
- ssGSEA揭示了SVEP1,MOXD1,SMOC2和COPD中激活的CD8T细胞之间的相关性,以及SVEP1与HF中26种免疫细胞类型之间的相关性.
- 凯格路径分析将WNT,VEGF和SPHINGOLIPID信号与这些共同敏感性基因联系起来.
结论:
- 确定了一组在COPD和HF中显著上调的基因小组.
- 四个基因 (SVEP1,MOXD1,SMOC2,GNB3) 对COPD-HF并发症具有高的诊断价值.
- 开发了一个名谱来评估这些关键基因的诊断潜力.
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