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使用UHPLC高分辨率质谱测量对代谢物进行大脑组织预处理策略的比较评估

Fang Guo¹, Xiaoxue Zhou², Yudie Ning¹

¹Shanxi Medical University, Jinzhong, Shanxi 030600, China; Key Laboratory of Forensic Toxicological Analysis, Ministry of Public Security, Jinzhong, Shanxi 030600, China.

Journal of neuroscience methods

|July 5, 2025

概括

优化大脑样本准备用于代谢学是至关重要的. 干研磨与单相提取相结合,为UHPLC-HRMS研究提供了卓越的代谢物覆盖率和可重复性.

科学领域:

神经科学是一个神经科学.
分析化学分析化学
生物化学生化学

背景情况:

人类大脑含有重要的代谢物,有助于理解大脑功能和疾病.
由于大脑的可液化性质,快速样本制备对于保持代谢概况至关重要.
物理均质化是有效地从大脑组织释放代谢物的必要条件.

研究的目的:

用高分辨率质谱测量来确定大脑代谢学最佳样本制备程序.
评估不同同质化和提取技术的有效性,以分析大脑代谢物.

主要方法:

评估了四种不同的同质化技术.
我们比较了三种不同的提取协议.
使用超高性能液态染色学与高分辨率质谱学 (UHPLC-HRMS) 进行分析.

主要成果:

与其他方法相比,干研磨均质化产生了最多的特征峰和最高峰区域.
单相提取显示出比双相和双相提取方法显著更好的可重复性.
单相和双相提取方法都显示出比双相提取更高的峰值区域,它们之间没有显著差异 (除了氨酸).

结论:

干磨和单相提取的组合建议用于大脑代谢学.
这种优化的方法提高了UHPLC-HRMS研究的代谢物覆盖率,可复制性和适应性.
这些发现为在代谢学研究中进行大脑组织分析提供了卓越的方法.

关键词:

脑中的大脑.代谢学代谢学代谢学预处理预处理在 UPLC Orbitrap Q Exactive Plus 中使用.

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