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温京液通过BDNF/TRKB/CREB通路对冷凝和静止PD产生镇痛作用

Xinhua Li¹, Xiaoyan Wang², Min Zhou²

¹Hebei international Joint Research Center for Dominant Diseases in Chinese Medicine and Acupuncture, Hebei University of Chinese Medicine.Shijiazhuang, Hebei, 050200, China; School of Acupuncture-Moxibustionand Tuina, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China; Hebei Key Laboratory of Integrative Medicine on Liver-Kidney Patterns, Institute of Integrative Medicine, College of Integrative Medicine, Hebei University of Chinese Medicine, Shijiazhuang, 050200, China.

Journal of ethnopharmacology

|July 5, 2025

概括

温京 (WJD) 通过抑制中央和外围疼痛敏感化来缓解大鼠的原发性经期障碍 (PD) 疼痛. 这种传统中医药调节BDNF/TrkB/CREB通路,为其在治疗PD的使用提供了科学依据.

科学领域:

药理学和中国传统医学
神经科学和疼痛研究研究
妇科和生殖健康妇科和生殖健康

背景情况:

文京 (Wenjing decoction,简称WJD) 是一种传统的中医药,用于治疗一次性经期障碍 (PD).
众所周知,WJD调节内分泌水平,减少炎症,改善微循环,缓解疼痛,但其机制尚不清楚.

研究的目的:

为了研究疼痛敏感化在PD中的作用.
在患有冷凝血和血静止综合征 (CCBS) 的PD大鼠中阐明WJD的止痛机制.
为WJD在PD的临床应用提供实验证据.

主要方法:

在WJD中UHPLC‒MS/MS确定了化合物.
建立了一种PD大鼠模型 (CCBS综合征),并用WJD或布洛芬治疗.
评估了CCBS得分,子宫微循环,血清因素,疼痛行为和组织形态学.
RNA-seq分析了PD病原和WJD机制.
评估了外围和中心疼痛敏感化标志物 (BDNF/TrkB/CREB通路).

主要成果:

在WJD中发现了33种化合物.
WJD缓解了CCBS综合征,疼痛行为,改善了子宫微循环,并减少了PD大鼠的炎症标志物.
RNA-seq显示WJD降低了与疼痛敏感化 (Trpv1,Bdnf,Pgf) 和神经炎症相关的基因.
在PD大鼠中,WJD治疗降低了BDNF,TRPV1,PTGFR和中央疼痛路径标记物 (BDNF/TrkB/CREB,CD11b,c-FOS) 的升高水平.

结论:

中央和外围疼痛敏感化是患有CCBS综合征的PD的关键.
通过降低hypothalamic BDNF/TrkB/CREB信号的调节,WJD抑制了中央微质和神经元的激活.
通过减少疼痛敏感度,WJD在PD老鼠中发挥了镇痛作用,支持其在PD的TCM中使用.

关键词:

在BDNF/TRKB/CREB信号通路中.寒冷凝固静止综合征 (CCBS) 是一种疾病.疼痛敏感化疼痛敏感化主要的经期障碍 (PD)这就是WJD的原因.

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