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基于光黄金纳米集群和p-nitrophenyl酸盐的性酸酶的快速和灵敏检测

So-Hee Kim¹, Chang Soon Huh¹, Moon-Moo Kim¹

¹Department of Cosmetics Engineering, and Graduate School of Applied Chemistry and Food Engineering, Dong-Eui University, Busan 47340, Republic of Korea.

Journal of bioscience and bioengineering

|July 5, 2025

概括

一种新的测定方法使用金纳米集群来检测性酸酶 (ALP) 活性. 这种基于光火的敏感方法对ALP相关疾病的临床诊断有前途.

科学领域:

生物化学生物化学
纳米技术纳米技术
分析化学分析化学

背景情况:

性酸酶 (ALP) 对于代谢过程至关重要,异常水平表明疾病.
目前对ALP的诊断方法可能缺乏敏感性或简单性.
金纳米集群 (AuNC) 为生物传感应用提供独特的光学特性.

研究的目的:

开发一种简单而敏感的基于光的试验,用于检测性酸酶 (ALP) 活性.
为了利用谷氨结合金纳米集群 (GSH-AuNCs) 和p-nitrophenyl酸盐 (pNPP) 来检测ALP.
为诊断目的研究光火机制.

主要方法:

使用UV-Vis光谱和传输电子显微镜 (TEM) 合成和表征谷联合金纳米集群 (GSH-AuNCs).
开发一种光灭试验,其中p-nitrophenyl酸盐 (pNPP) 和其产物p-nitrophenol (pNP) 灭GSH-AuNCs光.
使用二甲基硫酸盐聚烯胺凝电泳 (SDS-PAGE) 和在真实生物和植物样本中进行测试来验证试验.

主要成果:

通过TEM,GSH-AuNCs表现出600nm的特性吸收和统一的球形形态.
pNPP和pNP有效地灭了GSH-AuNCs的光,灭强度随着度的增加而增加.
该试验显示ALP的敏感检测范围为1.95-1000U/L,相关系数高 (0.976).

结论:

开发的基于GSH-AuNCs的测定方法提供了一种灵敏而简单的方法来检测性酸酶 (ALP) 活性.
光灭机制是有效的量化ALP水平在各种现实世界的样本,包括细胞溶解物,培养基和植物提取物.
这种测试对临床诊断和涉及ALP活动的生物医学研究具有重大潜力.

关键词:

性酸酶是一种性酸酶.光灭的光灭方式葡萄糖氨酸 (Glutathione) 是一种黄金纳米集群黄金纳米集群在p-Nitrophenyl 酸盐中.

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