通过H2S介导的蛋白质S-硫化调节了大米爆发真菌的传染性和自性
Hong Hu1, Mengyuan Qin1, Jiening Zhang1
1National Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Nature communications
|July 5, 2025
概括
硫化 (H2S) 通过调节蛋白S-硫化,对真菌感染至关重要. 过度的H2S通过损害自性来抑制真菌感染力,为疾病控制提供了一个目标.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 植物病理学 植物病理学
背景情况:
- 硫化 (H2S) 是一种信号分子,通过植物和哺乳动物的S-硫化调节细胞过程.
- 在真菌中,特别是植物病原菌中,H2S的功能和分子标尚未得到充分理解.
研究的目的:
- 调查H2S在大米爆发真菌Magnaporthe oryzae感染过程中的作用.
- 确定M. oryzae中H2S介导的S-硫化的分子标,并阐明底层的调节机制.
主要方法:
- 使用定量蛋白质组学来识别M. oryzae中的S-硫化蛋白质.
- 分析H2S水平对真菌感染力和自的影响.
- 位点定向突变发生,以调查特定的囊残留物在蛋白质功能中的作用.
主要成果:
- 由囊氨酸γ-酶 (CSE1) 合成的H2S对于M. oryzae感染至关重要.
- 过度的H2S通过S-硫化损害了自性,从而抑制了真菌感染力.
- 与自相关的蛋白质ATG18被确定为H2S标;在Cys78的S-硫化会影响其与酸-3酸盐的结合,影响自.
结论:
- 通过H2S介导的ATG18的S-硫化是控制M. oryzae.e.自的关键调节机制.
- 这项研究揭示了H2S在真菌发病过程中的新作用,并提供了利用H2S捐赠体控制真菌疾病的潜在策略.
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